Abstract

Amphotericin B (AmB) is an important antifungal agent available in the clinical practice with the action mechanism related to the inhibition of ergosterol molecule present in the fungal cell wall. Given this, in order to expand AmB knowledge, this review article gathers important information of the AmB physical, chemical, and biological properties. In addition, the main analytical methods for quantifying and determining the AmB were also reported in this review, such as high-performance liquid chromatography (HPLC), liquid chromatography, tandem mass spectrophotometry (LC-MS/MS), immunoenzymatic assay (ELISA), capillary zone electrophoresis (CE) stands out and among others. Based in this review article, the scientific community will have important information to choose the best method for analysis in their scientific or clinical research, providing greater security and reliability in the obtained results.

摘要

两性霉素B（AmB）是临床上可用的重要抗真菌剂，其作用机制与抑制真菌细胞壁中存在的麦角甾醇分子有关。鉴于此，为了扩展 AmB 知识，这篇评论文章收集了 AmB 物理、化学和生物学特性的重要信息。此外，本综述还报道了定量测定AmB的主要分析方法，如高效液相色谱法（HPLC）、液相色谱法、串联质谱法（LC-MS/MS）、免疫酶法（ELISA）等。 ，毛细管区带电泳 (CE) 脱颖而出等。根据这篇评论文章，科学界将获得重要信息，以便在其科学或临床研究中选择最佳分析方法，