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Expression profile analysis of circular RNAs in BmN cells (Bombyx mori) upon BmNPV infection.
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-09-02 , DOI: 10.1002/arch.21735 Shaolun Zhang 1 , Manman Shen 1, 2 , Haotong Yin 1 , Haoling Huang 1 , Tao Li 1 , Weiguo Zhao 1, 2 , Xijie Guo 1, 2 , Ping Wu 1, 2
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-09-02 , DOI: 10.1002/arch.21735 Shaolun Zhang 1 , Manman Shen 1, 2 , Haotong Yin 1 , Haoling Huang 1 , Tao Li 1 , Weiguo Zhao 1, 2 , Xijie Guo 1, 2 , Ping Wu 1, 2
Affiliation
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The disease caused by Bombyx mori nucleopolyhedrovirus (BmNPV) has always been difficult to control, resulting in tremendous economic losses in the sericulture industry. Although much has been learned about the impact of noncoding RNAs on pathogenesis, the role of circular RNA (circRNA) in insect immunity remains unclear. To explore circRNA regulation involved in BmNPV infection, we used transcriptome analysis of BmN cells with or without BmNPV infection to generate circRNA data set. A total of 444 novel circRNAs were identified in BmN cells, with 198 pervasively distributed both in the control group and BmNPV‐infection group. The host genes were enriched inMAPK signaling pathway, dorso–ventral axis formation, and ECM–receptor interaction, which were required for the normal larval growth. A total of 75 circRNAs were differentially expressed (DE) on BmNPV infection. Six downregulated circRNAs were validated by Sanger sequencing and qRT‐PCR. DEcircRNA–miRNA–DEmRNA network was constructed based on the six validated circRNAs. Pathway analysis indicated that the predicted target genes were mainly enriched in the metabolic pathway and immune‐related signaling pathway. Our results may provide a basis for further studies on circRNA function in BmN cells challenged by BmNPV infection and offer an insight into the molecular mechanism on silkworm–virus interaction.
中文翻译：
BmNPV感染后BmN细胞（Bombyx mori）中环状RNA的表达谱分析。
家蚕引起的疾病核多角体病毒（BmNPV）一直很难控制，在蚕桑业中造成了巨大的经济损失。尽管人们已经学到了许多关于非编码RNA对发病机理的影响的知识，但是环状RNA（circRNA）在昆虫免疫中的作用仍然不清楚。为了探索参与BmNPV感染的circRNA调控，我们使用了有或没有BmNPV感染的BmN细胞的转录组分析来生成circRNA数据集。在BmN细胞中总共鉴定出444种新颖的circRNA，在对照组和BmNPV感染组中普遍分布着198种。宿主基因丰富了正常幼虫生长所必需的MAPK信号传导途径，背腹轴形成和ECM受体相互作用。总共75个circRNA在BmNPV感染中被差异表达（DE）。通过Sanger测序和qRT-PCR验证了6个下调的circRNA。DEcircRNA–miRNA–DEmRNA网络是基于六个经过验证的circRNA构建的。途径分析表明，预测的靶基因主要富集在代谢途径和免疫相关的信号传导途径中。我们的研究结果可能为进一步研究在BmNPV感染的BmN细胞中circRNA功能提供基础，并为了解家蚕与病毒相互作用的分子机制提供了见识。
更新日期：2020-10-12
中文翻译：
BmNPV感染后BmN细胞（Bombyx mori）中环状RNA的表达谱分析。
家蚕引起的疾病核多角体病毒（BmNPV）一直很难控制，在蚕桑业中造成了巨大的经济损失。尽管人们已经学到了许多关于非编码RNA对发病机理的影响的知识，但是环状RNA（circRNA）在昆虫免疫中的作用仍然不清楚。为了探索参与BmNPV感染的circRNA调控，我们使用了有或没有BmNPV感染的BmN细胞的转录组分析来生成circRNA数据集。在BmN细胞中总共鉴定出444种新颖的circRNA，在对照组和BmNPV感染组中普遍分布着198种。宿主基因丰富了正常幼虫生长所必需的MAPK信号传导途径，背腹轴形成和ECM受体相互作用。总共75个circRNA在BmNPV感染中被差异表达（DE）。通过Sanger测序和qRT-PCR验证了6个下调的circRNA。DEcircRNA–miRNA–DEmRNA网络是基于六个经过验证的circRNA构建的。途径分析表明，预测的靶基因主要富集在代谢途径和免疫相关的信号传导途径中。我们的研究结果可能为进一步研究在BmNPV感染的BmN细胞中circRNA功能提供基础，并为了解家蚕与病毒相互作用的分子机制提供了见识。
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