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Detecting the nature and solving the crystal structure of a contaminant protein from an opportunistic pathogen.
Acta Crystallographica Section F ( IF 1.1 ) Pub Date : 2020-09-03 , DOI: 10.1107/s2053230x20010626 Riccardo Pederzoli 1 , Delia Tarantino 2 , Louise J Gourlay 2 , Antonio Chaves-Sanjuan 2 , Martino Bolognesi 2
Acta Crystallographica Section F ( IF 1.1 ) Pub Date : 2020-09-03 , DOI: 10.1107/s2053230x20010626 Riccardo Pederzoli 1 , Delia Tarantino 2 , Louise J Gourlay 2 , Antonio Chaves-Sanjuan 2 , Martino Bolognesi 2
Affiliation
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The unintentional crystallization of contaminant proteins in the place of target recombinant proteins is sporadically reported, despite the availability of stringent expression/purification protocols and of software for the detection of contaminants. Typically, the contaminant protein originates from the expression organism (for example Escherichia coli), but in rare circumstances contaminants from different sources have been reported. Here, a case of contamination from a Serratia bacterial strain that occurred while attempting to crystallize an unrelated protein from Burkholderia pseudomallei (overexpressed in E. coli) is presented. The contamination led to the unintended crystallization and structure analysis of a cyanase hydratase from a bacterial strain of the Serratia genus, an opportunistic enterobacterium that grows under conditions similar to those of E. coli and that is found in a variety of habitats, including the laboratory environment. In this context, the procedures that were adopted to identify the contaminant based on crystallographic data only are presented and the crystal structure of Serrata spp. cyanase hydratase is briefly discussed.
中文翻译：
检测机会性病原体污染蛋白的性质并解析其晶体结构。
尽管有严格的表达/纯化方案和用于检测污染物的软件，但偶尔有污染物蛋白无意结晶取代目标重组蛋白的报道。通常，污染物蛋白源自表达生物体（例如大肠杆菌），但在极少数情况下，也报道了来自不同来源的污染物。在此，介绍了一个沙雷氏菌菌株污染的案例，该案例是在尝试从拟鼻疽伯克霍尔德氏菌（在大肠杆菌中过度表达）中结晶不相关的蛋白质时发生的。污染导致了沙雷氏菌属细菌菌株中氰化酶水合酶的意外结晶和结构分析，沙雷氏菌属是一种机会性肠杆菌，其生长条件与大肠杆菌相似，存在于包括实验室在内的各种环境中。环境。在这种情况下，仅介绍了根据晶体学数据识别污染物所采用的程序以及锯缘石属的晶体结构。简要讨论氰化酶水合酶。
更新日期：2020-09-03
中文翻译：
检测机会性病原体污染蛋白的性质并解析其晶体结构。
尽管有严格的表达/纯化方案和用于检测污染物的软件，但偶尔有污染物蛋白无意结晶取代目标重组蛋白的报道。通常，污染物蛋白源自表达生物体（例如大肠杆菌），但在极少数情况下，也报道了来自不同来源的污染物。在此，介绍了一个沙雷氏菌菌株污染的案例，该案例是在尝试从拟鼻疽伯克霍尔德氏菌（在大肠杆菌中过度表达）中结晶不相关的蛋白质时发生的。污染导致了沙雷氏菌属细菌菌株中氰化酶水合酶的意外结晶和结构分析，沙雷氏菌属是一种机会性肠杆菌，其生长条件与大肠杆菌相似，存在于包括实验室在内的各种环境中。环境。在这种情况下，仅介绍了根据晶体学数据识别污染物所采用的程序以及锯缘石属的晶体结构。简要讨论氰化酶水合酶。
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