Plants use a variety of signals to control root development, including in modifying root development in response to nutrient stress. For example, in response to nitrogen (N) stress, plants dramatically modulate root development, including the formation of N-fixing nodules in legumes. Recently, specific CLE peptides and/or receptors important for their perception, including CLV1 and CLV2, have been found to play roles in root development, including in response to N supply. In the legume Medicago truncatula, this response also appears to be influenced by RDN1, a member of the hydroxyproline O-arabinosyltransferase (HPAT) family which can modify specific CLE peptides. However, it is not known if this signalling pathway plays a central role in root development across species, and in particular root responses to N. In this study, we systematically examined the role of the CLV signalling pathway genes in root development of the legume pea (Pisum sativum) and non-legume tomato (Solanum lycopersicum) using a mutant-based approach. This included a detailed examination of root development in response to N in tomato mutants disrupted in CLV1- or CLV2-like genes or HPAT family member FIN. We found no evidence for a role of these genes in pea seedling root development. Furthermore, the CLV1-like FAB gene did not influence tomato root development, including the root response response to N supply. In contrast, both CLV2 and the HPAT gene FIN appear to positively influence root size in tomato but do not mediate root responses to N. These results suggest the function of these genes may vary somewhat in different species, including the N regulation of root architecture. This article is protected by copyright. All rights reserved.

中文翻译：

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CLV1、CLV2 和 HPAT 同源物在根发育氮调节中的作用

植物使用多种信号来控制根系发育，包括在响应养分胁迫时改变根系发育。例如，在应对氮 (N) 胁迫时，植物会显着调节根系发育，包括在豆科植物中形成固氮根瘤。最近，已发现对其感知很重要的特定 CLE 肽和/或受体，包括 CLV1 和 CLV2，在根发育中发挥作用，包括响应 N 供应。在豆科植物苜蓿中，这种反应似乎也受到 RDN1 的影响，RDN1 是羟脯氨酸 O-阿拉伯糖基转移酶 (HPAT) 家族的成员，可以修饰特定的 CLE 肽。然而，尚不清楚这种信号通路是否在不同物种的根发育中发挥核心作用，尤其是根对 N 的反应。在这项研究中，我们使用基于突变的方法系统地检查了 CLV 信号通路基因在豆科豌豆 (Pisum sativum) 和非豆科番茄 (Solanum lycopersicum) 根发育中的作用。这包括对 CLV1 或 CLV2 样基因或 HPAT 家族成员 FIN 中破坏的番茄突变体中 N 响应的根发育进行详细检查。我们没有发现这些基因在豌豆幼苗根发育中的作用的证据。此外，CLV1 样 FAB 基因不影响番茄根发育，包括根对 N 供应的反应。相比之下，CLV2 和 HPAT 基因 FIN 似乎都对番茄的根大小产生积极影响，但不会介导根对 N 的反应。这些结果表明这些基因的功能在不同物种中可能有所不同，包括根结构的 N 调节。本文受版权保护。版权所有。