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Improved lignocellulose saccharification of a Miscanthus reddish stem mutant induced by heavy‐ion irradiation
Global Change Biology Bioenergy ( IF 5.9 ) Pub Date : 2020-09-02 , DOI: 10.1111/gcbb.12748 Congpeng Wang 1 , Guo He 2, 3 , Jie Meng 1 , Shumin Wang 2 , Yingzhen Kong 4 , Jianxiong Jiang 5 , Ruibo Hu 2 , Gongke Zhou 1
Global Change Biology Bioenergy ( IF 5.9 ) Pub Date : 2020-09-02 , DOI: 10.1111/gcbb.12748 Congpeng Wang 1 , Guo He 2, 3 , Jie Meng 1 , Shumin Wang 2 , Yingzhen Kong 4 , Jianxiong Jiang 5 , Ruibo Hu 2 , Gongke Zhou 1
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Miscanthus is a promising lignocellulosic bioenergy crop for the production of cellulosic ethanol. The recalcitrance of lignocellulose hampers the efficient conversion of Miscanthus biomass to fermentable sugars. Therefore, screening of Miscanthus germplasms with enhanced lignocellulose saccharification efficiency is of important significance to the cellulosic ethanol production from Miscanthus biomass. In this study, we utilize heavy‐ion irradiation mutagenesis to establish a mutant library of Miscanthus lutarioriparius, and screened a reddish stem (rs) mutant with improved lignocellulose saccharification efficiency. The rs mutant was characterized by a noticeable reddish coloration in various lignified cell walls in phloem and vascular bundle sheath, whereas the biomass yield was not compromised. The reddish coloration was attributed to the reduced incorporation of several phenolic compounds including 3‐caffeoylquinic acid, dihydroflavonol and proanthocyanin A, which shared common precursors with lignin monolignol biosynthesis via the phenylpropanoid pathway. Correspondingly, the lignin content was decreased by 10.2% and the cellulose content was concomitantly increased by 9.5% in rs mutant compared with the control plant. Meanwhile, the saccharification efficiency was substantially improved by 22.4%–25.6% in rs mutant compared to the control, when the lignocellulosic biomass was pretreated with or without 1.5% H2SO4. The rs mutant identified here holds a potential utilization as an ideal candidate feedstock in cellulosic bioethanol production.
中文翻译：
重离子辐射诱导的芒草微红色茎突变体的木质纤维素糖化改善
芒草是有前景的木质纤维素生物能源作物，用于生产纤维素乙醇。木质纤维素的顽固阻碍了芒草生物质向可发酵糖的有效转化。因此，筛选具有提高的木质纤维素糖化效率的芒草种质对于由芒草生物质生产纤维素乙醇具有重要意义。在这项研究中，我们利用重离子辐射诱变建立了Miscanthus lutarioriparius突变体文库，并筛选了具有改善的木质纤维素糖化效率的红色茎（rs）突变体。该RS突变体的特征是在韧皮部和维管束鞘的各种木质化细胞壁上显着的红色，而生物量的产量没有受到损害。偏红的颜色归因于几种酚类化合物的掺入减少，包括3-咖啡酰奎宁酸，二氢黄酮醇和原花青素A，它们通过苯丙烷类途径与木质素单木酚生物合成共有共同的前体。相应地，与对照植物相比，rs突变体中木质素含量降低了10.2％，纤维素含量相应地提高了9.5％。同时，糖化效率在rs中大大提高了22.4％–25.6％当使用或不使用1.5％H 2 SO 4预处理木质纤维素生物质时，与对照相比，该突变体与对照相比。此处鉴定的rs突变体具有潜在的利用潜力，可作为纤维素生物乙醇生产中的理想候选原料。
更新日期：2020-09-02
中文翻译：
重离子辐射诱导的芒草微红色茎突变体的木质纤维素糖化改善
芒草是有前景的木质纤维素生物能源作物，用于生产纤维素乙醇。木质纤维素的顽固阻碍了芒草生物质向可发酵糖的有效转化。因此，筛选具有提高的木质纤维素糖化效率的芒草种质对于由芒草生物质生产纤维素乙醇具有重要意义。在这项研究中，我们利用重离子辐射诱变建立了Miscanthus lutarioriparius突变体文库，并筛选了具有改善的木质纤维素糖化效率的红色茎（rs）突变体。该RS突变体的特征是在韧皮部和维管束鞘的各种木质化细胞壁上显着的红色，而生物量的产量没有受到损害。偏红的颜色归因于几种酚类化合物的掺入减少，包括3-咖啡酰奎宁酸，二氢黄酮醇和原花青素A，它们通过苯丙烷类途径与木质素单木酚生物合成共有共同的前体。相应地，与对照植物相比，rs突变体中木质素含量降低了10.2％，纤维素含量相应地提高了9.5％。同时，糖化效率在rs中大大提高了22.4％–25.6％当使用或不使用1.5％H 2 SO 4预处理木质纤维素生物质时，与对照相比，该突变体与对照相比。此处鉴定的rs突变体具有潜在的利用潜力，可作为纤维素生物乙醇生产中的理想候选原料。
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