Thyroid cancer is the most common endocrine carcinoma, with papillary thyroid carcinoma (PTC) accounting for 80%‐90% of thyroid cancers. Accumulating studies reported that mitochondria plays an important role in the regulation of cell proliferation. ALDH5A1, may function as an oncogene or tumour suppressor in various human cancers, and the role of ALDH5A1 in PTC is still unclear. The aim of this study was to investigate the clinical significance of ALDH5A1 expression and its functions in PTC. In this present study, we studied ALDH5A1 expression on primary papillary thyroid carcinoma (PTC) in The Cancer Genome Atlas (TCGA) database. Results showed that the levels of ALDH5A1 were found positively correlated with tumour stage, metastasis, lymph node stage, and higher levels of ALDH5A1 demonstrated poor disease‐free survival (DFS). Immunohistochemistry (IHC) revealed that significantly higher expression of ALDH5A1 was found in PTC tissues. On the other hand, knockdown of ALDH5A1 significantly inhibited PTC cell proliferation, migration and invasion detection found the migration and invasion of cells also were hindered when ALDH5A1 level was reduced. The knockdown of ALDH5A1 inhibited the expression of Vimentin and promoted the expression of E‐cadherin. In brief, knockdown of ALDH5A1may act as a novel molecular target for the prevention and treatment of PTC.

中文翻译：

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ALDH5A1充当肿瘤启动子，对甲状腺乳头状癌有预后影响

甲状腺癌是最常见的内分泌癌，乳头状甲状腺癌（PTC）占甲状腺癌的80％-90％。越来越多的研究报告说，线粒体在调节细胞增殖中起着重要作用。ALDH5A1可能在多种人类癌症中起癌基因或抑癌作用，目前尚不清楚ALDH5A1在PTC中的作用。这项研究的目的是调查ALDH5A1表达及其在PTC中的功能的临床意义。在本研究中，我们在癌症基因组图谱（TCGA）数据库中研究了ALDH5A1在原发性甲状腺乳头状甲状腺癌（PTC）上的表达。结果表明，ALDH5A1的水平与肿瘤的分期，转移，淋巴结分期呈正相关，而较高的ALDH5A1的无病生存期（DFS）则较差。免疫组织化学（IHC）显示，在PTC组织中发现ALDH5A1的表达明显较高。另一方面，敲低ALDH5A1可显着抑制PTC细胞的增殖，迁移和侵袭检测发现当ALDH5A1水平降低时，细胞的迁移和侵袭也受到阻碍。敲低ALDH5A1抑制波形蛋白的表达并促进E-钙黏着蛋白的表达。简而言之，敲低ALDH5A1可以作为预防和治疗PTC的新型分子靶标。敲低ALDH5A1抑制波形蛋白的表达并促进E-钙黏着蛋白的表达。简而言之，敲低ALDH5A1可以作为预防和治疗PTC的新型分子靶标。敲低ALDH5A1抑制波形蛋白的表达并促进E-钙黏着蛋白的表达。简而言之，敲低ALDH5A1可以作为预防和治疗PTC的新型分子靶标。