Linoleic acid inhibits Pseudomonas aeruginosa biofilm formation by activating diffusible signal factor-mediated quorum sensing.,Biotechnology and Bioengineering

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Linoleic acid inhibits Pseudomonas aeruginosa biofilm formation by activating diffusible signal factor-mediated quorum sensing.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-09-03 , DOI: 10.1002/bit.27552
Han-Shin Kim ₁ , Eunji Cha ₂ , So-Young Ham ₂ , Jeong-Hoon Park ₃ , SangJin Nam ₄ , Hongmok Kwon ₄ , Youngjoo Byun _{4,

5} , Hee-Deung Park _{2,

6}

Affiliation

Bacterial biofilm formation causes serious problems in various fields of medical, clinical, and industrial settings. Antibiotics and biocide treatments are typical methods used to remove bacterial biofilms, but biofilms are difficult to remove effectively from surfaces due to their increased resistance. An alternative approach to treatment with antimicrobial agents is using biofilm inhibitors that regulate biofilm development without inhibiting bacterial growth. In the present study, we found that linoleic acid (LA), a plant unsaturated fatty acid, inhibits biofilm formation under static and continuous conditions without inhibiting the growth of Pseudomonas aeruginosa. LA also influenced the bacterial motility, extracellular polymeric substance production, and biofilm dispersion by decreasing the intracellular cyclic diguanylate concentration through increased phosphodiesterase activity. Furthermore, quantitative gene expression analysis demonstrated that LA induced the expression of genes associated with diffusible signaling factor‐mediated quorum sensing that can inhibit or induce the dispersion of P. aeruginosa biofilms. These results suggest that LA is functionally and structurally similar to a P. aeruginosa diffusible signaling factor (cis‐2‐decenoic acid) and, in turn, act as an agonist molecule in biofilm dispersion.

中文翻译：

亚油酸通过激活可扩散信号因子介导的群体感应来抑制铜绿假单胞菌生物膜的形成。

细菌生物膜的形成在医学、临床和工业环境的各个领域引起了严重的问题。抗生素和杀菌剂处理是用于去除细菌生物膜的典型方法，但生物膜由于其抵抗力增加而难以从表面有效去除。用抗微生物剂治疗的另一种方法是使用生物膜抑制剂，在不抑制细菌生长的情况下调节生物膜的发展。在本研究中，我们发现亚油酸 (LA) 是一种植物不饱和脂肪酸，可在静态和连续条件下抑制生物膜形成，而不会抑制铜绿假单胞菌的生长. LA 还通过增加磷酸二酯酶活性来降低细胞内环状二鸟苷酸浓度，从而影响细菌运动、细胞外聚合物的产生和生物膜分散。此外，定量基因表达分析表明，LA 诱导与可扩散信号因子介导的群体感应相关的基因表达，该群体感应可以抑制或诱导铜绿假单胞菌生物膜的分散。这些结果表明 LA 在功能和结构上与铜绿假单胞菌可扩散信号因子（顺式‐2-癸烯酸）相似，进而在生物膜分散中充当激动剂分子。

更新日期：2020-09-03

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