Controlled Deposition of 3D Matrices to Direct Single Cell Functions,Advanced Science

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Controlled Deposition of 3D Matrices to Direct Single Cell Functions
Advanced Science ( IF 14.3 ) Pub Date : 2020-09-03 , DOI: 10.1002/advs.202001066
Sing Wan Wong _{1,

2} , Stephen Lenzini _{1,

2} , Raymond Bargi _{1,

2} , Zhe Feng ₃ , Celine Macaraniag ₂ , James C Lee ₂ , Zhangli Peng ₂ , Jae-Won Shin _{1,

2}

Affiliation

Advances in engineered hydrogels reveal how cells sense and respond to 3D biophysical cues. However, most studies rely on interfacing a population of cells in a tissue‐scale bulk hydrogel, an approach that overlooks the heterogeneity of local matrix deposition around individual cells. A droplet microfluidic technique to deposit a defined amount of 3D hydrogel matrices around single cells independently of material composition, elasticity, and stress relaxation times is developed. Mesenchymal stem cells (MSCs) undergo isotropic volume expansion more rapidly in thinner gels that present an Arg‐Gly‐Asp integrin ligand. Mathematical modeling and experiments show that MSCs experience higher membrane tension as they expand in thinner gels. Furthermore, thinner gels facilitate osteogenic differentiation of MSCs. By modulating ion channels, it is shown that isotropic volume expansion of single cells predicts intracellular tension and stem cell fate. The results suggest the utility of precise microscale gel deposition to control single cell functions.

中文翻译：

控制沉积 3D 基质以指导单细胞功能

工程水凝胶的进展揭示了细胞如何感知和响应 3D 生物物理线索。然而，大多数研究依赖于在组织规模的块状水凝胶中连接细胞群，这种方法忽视了单个细胞周围局部基质沉积的异质性。开发了一种液滴微流体技术，可在单细胞周围沉积确定数量的 3D 水凝胶基质，与材料成分、弹性和应力松弛时间无关。间充质干细胞 (MSC) 在呈现 Arg-Gly-Asp 整联蛋白配体的较薄凝胶中更快地进行各向同性体积膨胀。数学模型和实验表明，间充质干细胞在更薄的凝胶中扩张时会经历更高的膜张力。此外，较薄的凝胶有利于 MSC 的成骨分化。通过调节离子通道，单细胞的各向同性体积膨胀可预测细胞内张力和干细胞命运。结果表明精确的微型凝胶沉积可用于控制单细胞功能。

更新日期：2020-10-22

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