Effect of the photodegradation of chemotherapeutic agent methotrexate on the denaturation of human serum albumin has been studied by differential scanning calorimetry and fluorimetric measurements. Photoluminescence studies highlighted entropy driven binding of both methotrexate molecules and its degradation products to the albumin molecules. The calorimetric measurements evaluated by the Kissinger method show elevated activation energy in the presence of methotrexate. Similar but moderated enhancement of the activation energy was obtained in the presence of the photodegradation products of methotrexate. These results highlight stabilization of the human serum albumin by the methotrexate drug which finding may contribute to fine tuning of methotrexate applications in therapy.

通过差示扫描量热法和荧光法研究了化学治疗剂甲氨蝶呤光降解对人血清白蛋白变性的影响。光致发光研究强调了甲氨蝶呤分子及其降解产物与白蛋白分子的熵驱动结合。通过基辛格方法评估的量热法测量结果表明，在存在甲氨蝶呤的情况下活化能升高。在甲氨蝶呤的光降解产物的存在下，活化能得到类似但适度的增强。这些结果突出了氨甲蝶呤药物对人血清白蛋白的稳定作用，这一发现可能有助于调整甲氨蝶呤在治疗中的应用。