The detection of characteristics associated with plant growth promotion has been studied frequently on bacteria and some of these detection methods were also used on yeasts. Sometimes, these methods, designed for prokaryotes, were used with no prior analysis regarding growth and production on eukaryotes. The aim of the present study was to assess and select the best suitable media for the detection of auxin-like compound production and inorganic-P solubilization on yeast strains. Already published media and new formulations, as well as yeasts with different genetic backgrounds were used for the comparison. Media were selected based on the adequate growth of yeast strains and reliable recognition of the studied features either by an easy detection of the metabolite (color or halo production) or simple medium preparation (low number of reagent and regular autoclaving). We propose here the use of the new DEV medium with glucose and with tryptophan to identify auxin-like compounds producers; and CPM medium (a variation from Custer's Chalk Medium) as base medium to identify yeasts capable of inorganic-P solubilization.

与植物生长促进相关的特征检测经常在细菌上进行研究，其中一些检测方法也用于酵母。有时，这些为原核生物设计的方法在使用之前没有对真核生物的生长和生产进行分析。本研究的目的是评估和选择最合适的培养基来检测酵母菌株的生长素样化合物的产生和无机磷的溶解。已经发表的媒体和新配方，以及具有不同遗传背景的酵母被用于比较。培养基的选择基于酵母菌株的充分生长和研究特征的可靠识别，通过代谢物的简单检测（颜色或晕产生）或简单的培养基制备（少量试剂和常规高压灭菌）。我们在此建议使用含有葡萄糖和色氨酸的新 DEV 培养基来鉴定生长素样化合物的生产者；和 CPM 培养基（Custer's Chalk 培养基的变体）作为基础培养基来鉴定能够溶解无机磷的酵母菌。