Pea3 proteins belong to a subfamily of the E-twentysix (ETS) domain superfamily of transcription factors, which play various roles during development. Polyoma Enhancer-Activator 3 (Pea3) proteins Pea3, ERM and Er81 are particularly involved in tissues with branching morphogenesis, including kidney, lung, mammary gland and nervous system development. A recent transcriptomic study on novel targets of Pea3 transcription factor revealed various axon guidance and nervous system development related targets, supporting a role of Pea3 proteins in motor neuron connectivity, as well as novel targets in signaling pathways involved in synaptic plasticity. This study focuses on the expression of Pea3 family members in hippocampal neurons, and regulation of putative Pea3 targets in Pea3-overexpressing cell lines and following induction of long-term potentiation or seizure in vivo. We show that Pea3 proteins are expressed in hippocampus in both neuronal and non-neuronal cells, and that Pea3 represses Elk-1 but activates Prkca and Nrcam expression in hippocampal cell lines. We also show that mRNA and protein levels of Pea3 family members are differentially regulated in the dentate gyrus and CA1 region upon MECS stimulation, but not upon LTP induction.

Pea3蛋白属于E-二十六个（ETS）域转录因子超家族的亚家族，在发育过程中发挥多种作用。多瘤增强子激活剂3（Pea3）蛋白Pea3，ERM和Er81特别参与具有分支形态发生的组织，包括肾脏，肺，乳腺和神经系统发育。最近对Pea3转录因子新靶标的转录组学研究揭示了各种轴突导向和神经系统发育相关靶标，支持了Pea3蛋白在运动神经元连通性中的作用，以及在涉及突触可塑性的信号通路中的新靶标。这项研究的重点是Pea3家族成员在海马神经元中的表达，体内。我们显示，Pea3蛋白在神经元和非神经元细胞的海马中表达，并且Pea3抑制Elk-1，但激活海马细胞系中的Prkca和Nrcam表达。我们还显示，在MECS刺激下，齿状回和CA1区的Pea3家族成员的mRNA和蛋白水平受到差异调节，而LTP诱导则没有。