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Xanthan gum as a carrier for bacterial cell entrapment: developing a novel immobilised biocatalyst
Biomaterials Advances ( IF 5.5 ) Pub Date : 2020-09-03 , DOI: 10.1016/j.msec.2020.111474
Anna Dzionek , Danuta Wojcieszyńska , Małgorzata Adamczyk-Habrajska , Jerzy Karczewski , Izabela Potocka , Urszula Guzik

Xanthan gum (XAN) is a widely used polysaccharide in various industries. Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen. The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%. A negative impact of entrapment was noted for strain Planococcus sp. S5, which showed a survival rate in the 93–51% range. To achieve good mechanical properties of the composites, they were additionally hardened using polydopamine (PDA). XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules. Analysis of the survival rate during storage at 4°C in 0.9% NaCl showed that, after 35 days, 98–99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive. This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora. Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.



中文翻译:

黄原胶作为细菌细胞包裹的载体:开发一种新型的固定化生物催化剂

黄原胶(XAN)是各种行业中广泛使用的多糖。由于其独特的性质,在这项研究中,尝试采用黄原胶交联的方法来包埋能够生物降解萘普生的细菌细胞。对于苏云金芽孢杆菌B1(2015b)细胞,开发的程序被证明是完全中性的,其存活率为99%。注意到包埋对平板球菌有不利影响sp。S5,存活率在93-51%之间。为了获得复合材料的良好机械性能,还使用聚多巴胺(PDA)对其进行了硬化。XAN / PDA复合材料在很宽的pH范围内都显示出高稳定性,其吸附能力包括阳离子和阴离子分子。在0.9%NaCl中于4°C储存期间的存活率分析表明,在35天后,XAN / PDA中捕获的B1(2015b)B1(2015b)和S5细胞中有98%至99%仍然存活。这项研究还介绍了使用XAN / PDA / B1(2015b)在带有自生菌群的滴滤池中进行萘普生生物降解的结果。因此,由于药物生物降解的显着加速(在14天中达到1 mg / L)和去除了化学需氧量,

更新日期:2020-09-03
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