Novel artificial tissues with potential usefulness in local-based therapies have been generated by tissue engineering using magnetic-responsive nanoparticles (MNPs). In this study, we performed a comprehensive in vivo characterization of bioengineered magnetic fibrin-agarose tissue-like biomaterials. First, in vitro analyses were performed and the cytocompatibility of MNPs was demonstrated. Then, bioartificial tissues were generated and subcutaneously implanted in Wistar rats and their biodistribution, biocompatibility and functionality were analysed at the morphological, histological, haematological and biochemical levels as compared to injected MNPs. Magnetic Resonance Image (MRI), histology and magnetometry confirmed the presence of MNPs restricted to the grafting area after 12 weeks. Histologically, we found a local initial inflammatory response that decreased with time. Structural, ultrastructural, haematological and biochemical analyses of vital organs showed absence of damage or failure. This study demonstrated that the novel magnetic tissue-like biomaterials with improved biomechanical properties fulfil the biosafety and biocompatibility requirements for future clinical use and support the use of these biomaterials as an alternative delivery route for magnetic nanoparticles.

通过使用磁响应纳米粒子（MNP）进行组织工程，已经产生了在局部疗法中具有潜在用途的新型人造组织。在这项研究中，我们对生物工程磁性纤维蛋白琼脂糖组织样生物材料进行了全面的体内表征。一，体外进行了分析，并证明了MNP的细胞相容性。然后，生成生物人工组织并皮下植入Wistar大鼠，并在形态，组织学，血液学和生化水平上与注射的MNP进行比较，分析其生物分布，生物相容性和功能。磁共振图像（MRI），组织学和磁力计证实12周后MNP限制在移植区域。从组织学上看，我们发现局部的初始炎症反应随时间减少。生命器官的结构，超微结构，血液学和生化分析显示没有损伤或衰竭。