Biotransformation of natural products to the natural flavoring, gamma-decalactone (GDL), has attracted considerable attention. However, improving its yield is challenging due to its high feedback inhibition of yeast cells, which lowers the productivity of the biotransformation process. In this study, we compared two in situ separation processes established by adding either resin (HZ-816) or cyclopentasiloxane (DC345) to a biotransformation medium and investigated their efficiency and effect on yeast metabolism. Compared with a control, yields from the medium with HZ-816 and DC345 increased by 140% and 175%, respectively. However, after 84 h of biotransformation, the protein leakage in the medium with HZ-816 and DC345 was respectively 2.04 times and 1.43 times that of the control. Meanwhile, the mortality of yeast cells was 32.8% and 24.0% in the medium with HZ-816 and DC345, respectively, whereas that in the control was 20.1%. Our findings indicate that a cyclase is involved in the final step of the biotransformation. The activity of the yeast cyclase in the DC345 system was 3.33 times greater than that in the HZ-816 system. The DC345 system was superior to the HZ-816 resin system in this separation process because its yield was 30.8% greater and it had less cellular damage. Thus, we showed that the DC345 system has potential as a new separation system for the production of GDL by biotransformation.

天然产物生物转化为天然香料γ-癸内酯（GDL）引起了人们的广泛关注。然而，由于其对酵母细胞的高反馈抑制，降低了生物转化过程的生产率，提高其产量具有挑战性。在本研究中，我们比较了通过向生物转化培养基中添加树脂 (HZ-816) 或环五硅氧烷 (DC345) 建立的两种原位分离工艺，并研究了它们的效率和对酵母代谢的影响。与对照相比，含有 HZ-816 和 DC345 的培养基的产量分别增加了 140% 和 175%。然而，生物转化84 h后，HZ-816和DC345培养基中的蛋白渗漏量分别是对照的2.04倍和1.43倍。同时，在含有HZ-816和DC345的培养基中，酵母细胞的死亡率分别为32.8%和24.0%，而对照培养基中的死亡率为20.1%。我们的研究结果表明环化酶参与生物转化的最后一步。 DC345系统中酵母环化酶的活性是HZ-816系统中的3.33倍。在此分离过程中，DC345 系统优于 HZ-816 树脂系统，因为其产量高出 30.8%，并且细胞损伤较小。因此，我们表明 DC345 系统具有作为生物转化生产 GDL 的新分离系统的潜力。