Abstract Fast methods for milk analysis are internationally desired, and, in this study, a new method for milk unsaturated fatty acids (UFAs) is proposed, using capillary zone electrophoresis with direct detection in the ultraviolet region. Run time analysis was 4 min, and sample preparation comprised only a saponification reaction, which indicates the suitability of the method for UFA monitoring in routine analyses by industries and milk researchers. Milk powder samples submitted to different oxygen content (0.3–20.4%) were analysed after ten months of storage to demonstrate method applicability, aiming to evaluate differences in UFA profiles, the most prone to lipid oxidation. No significant difference was noticed within the 95% confidence interval when comparing different samples. Limits of detection (mmol L−1) for UFAs were 0.040 (C18:1 n-9 cis); 0.014 (C18:2 n-6) and 0.010 (C18:3 n-3). Limits of quantification were 0.132, 0.046, and 0.034 mmol L−1, respectively.

中文翻译：

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牛奶中主要不饱和脂肪酸筛选的毛细管电泳方法

摘要 牛奶分析的快速方法是国际上需要的，在本研究中，提出了一种牛奶不饱和脂肪酸 (UFA) 的新方法，使用毛细管区带电泳在紫外区直接检测。运行时间分析为 4 分钟，样品制备仅包含皂化反应，这表明该方法适用于工业和牛奶研究人员的常规分析中的 UFA 监测。储存 10 个月后，对不同含氧量 (0.3–20.4%) 的奶粉样品进行分析，以证明方法的适用性，旨在评估最容易发生脂质氧化的 UFA 谱的差异。比较不同样品时，在 95% 置信区间内没有发现显着差异。UFA 的检测限 (mmol L-1) 为 0.040 (C18: 1 n-9 顺式）；0.014 (C18:2 n-6) 和 0.010 (C18:3 n-3)。定量限分别为 0.132、0.046 和 0.034 mmol L-1。