In chronic granulomatous disease (CGD) patients, reactive oxygen species (ROS) production by neutrophils is impaired. So, they are susceptible to infections. Studies showed that, mesenchymal stem cells (MSCs) have protective effects on the function of neutrophils and an approach that MSCs use to apply their effects, is secreting soluble factors and exosomes. So, we investigated the effects of MSC-exosomes and MSC-conditioned media (MSC-CM) on the function and apoptosis of neutrophils in CGD patients.

In this study, neutrophils were isolated from healthy donors and CGD patients and then incubated with exosomes or CM that were prepared from MSCs. Then, neutrophil respiratory burst, apoptosis and phagocytosis capacity were evaluated by NBT assay, Annexin V-PI method and Giemsa staining.

It was demonstrated that both MSC-exosomes and CM could improve the phagocytosis capacity and ROS production of neutrophils in CGD patients and healthy donors. In contrast to the healthy group, in CGD patients, exosomes significantly reduced the percentage of viable neutrophils.

This report indicated that MSC exosomes and CM could increase the function of the neutrophils isolated from CGD patients. But decreasing the number of the living cells is one of the limitations of them. However, it is hoped that this intervention will be developed in future studies to minimize its limitations.

在慢性肉芽肿病（CGD）患者中，中性粒细胞产生的活性氧（ROS）受到损害。因此，它们容易感染。研究表明，间充质干细胞（MSCs）对中性粒细胞的功能具有保护作用，并且MSCs发挥其作用的一种方法是分泌可溶性因子和外泌体。因此，我们研究了MSC外泌体和MSC条件培养基（MSC-CM）对CGD患者中性粒细胞功能和细胞凋亡的影响。

在这项研究中，嗜中性粒细胞从健康的供体和CGD患者中分离出来，然后与由MSC制备的外泌体或CM一起孵育。然后，通过NBT测定，膜联蛋白V-PI法和Giemsa染色评价中性粒细胞的呼吸爆发，凋亡和吞噬能力。

结果表明，MSC外泌体和CM均可改善CGD患者和健康供体的中性粒细胞的吞噬能力和ROS的产生。与健康组相比，在CGD患者中，外泌体显着降低了存活的中性粒细胞百分比。

该报告表明，MSC外泌体和CM可以增强从CGD患者中分离出的中性粒细胞的功能。但是减少活细胞的数量是它们的局限之一。但是，希望在将来的研究中开发这种干预措施，以最大程度地减少其局限性。