Abstract

It was found that nickel particles electrodeposited on the surface of planar carbon electrodes (PE; Ni–PE) exhibit catalytic activity in the electrooxidation of glucose, insulin, and cholesterol (CS) in alkaline media, and cobalt particles (Co–PE), in the oxidation of CS, insulin, and uric acid (UA) in neutral media. In recording an analytical signal in a system of sequential-injection analysis with amperometric detection, the bilogarithmic dependence of current on the analyte concentration on a Ni–PE electrode is linear in the range from 5 × 10^–6 to 5 × 10^–2 M for glucose, from 1 × 10^–6 to 1 × 10^–4 M for insulin, and from 5 × 10^–6 to 5 × 10^–3 M for CS. On the Co–PE electrode, it is linear from 5 × 10^–7 to 5 × 10^–3 M for CS, from 5 × 10^–8 to 5 × 10^–4 M for insulin, and from 5 × 10^–8 to 5 × 10^–3 M for UA. It is shown that the use of a two-detector system and modified double planar electrodes ensures the selective determination of the studied biologically active compounds in blood serum.

中文翻译：

---

镍和钴微粒修饰电极上的顺序注射安培法测定葡萄糖，胰岛素，胆固醇和尿酸

摘要

发现在碱性介质中，电沉积在平面碳电极（PE ; Ni–PE）表面的镍颗粒在葡萄糖，胰岛素和胆固醇（CS）的电氧化中表现出催化活性，而钴颗粒（Co–PE）中性介质中CS，胰岛素和尿酸（UA）的氧化 在具有安培检测的顺序注射分析系统中记录分析信号时，电流对Ni-PE电极上分析物浓度的双对数依赖性在5×10 ^–6到5×10 ^–2 M范围内是线性的用于葡萄糖，从1×10 ^–6到1×10 ^–4M为胰岛素，CS为5×10 ^–6到5×10 ^–3M。上共同PE电极，它是线性的，从5×10 ^-7〜5×10 ^-3 M代表CS，从5×10 ^-8〜5×10 ^-4 M代表胰岛素，和从5×10 ^-8至5×10 ^-3 M表示UA。结果表明，使用两个检测器系统和改进的双平面电极可确保对研究的血清中生物活性化合物进行选择性测定。