As rhizobacteria have extensively been studied for the production of compounds with biocontrol properties, methods to enhance its production are very important. In this study, nanotechnological method to modulate the rhamnolipid production by Pseudomonas sp. K6 has been demonstrated. For this, Pseudomonas sp. K6 was cultured with different concentrations of chitosan nanoparticles (CNPs) and gold nanoparticles (AuNPs). The rhamnolipid production was further analyzed by CTAB- methylene blue agar assay and also by LC-MS/MS analysis. From the LC-MS/MS result, induction of dirhamnolipid production in K6 could be confirmed when cultured with CNPs (1, 5 and 10 mg/mL) and AuNPs (10, 25, 50 and 100 µg/mL). The monorhamnolipid production by K6 was observed to get enhanced when the K6 culture was supplemented with 2.5 mg/mL CNPs and 10 µg/mL AuNPs. Also, in planta study confirmed the biocontrol ability of rhamnolipid as it suppressed the Sclerotium rolfsii infection in Vigna unguiculata plants. As the rhamnolipids have versatile applications in the agricultural field, the nano-based approach to enhance its production from the biocontrol organism is significant.

中文翻译：

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假单胞菌属农用鼠李糖脂谱的调节。K6 由于补充了壳聚糖和金纳米粒子

由于根际细菌已被广泛研究用于生产具有生物防治特性的化合物，因此提高其生产的方法非常重要。在这项研究中，纳米技术方法调节假单胞菌产生的鼠李糖脂。K6 已被证明。为此，假单胞菌属。K6 与不同浓度的壳聚糖纳米颗粒 (CNPs) 和金纳米颗粒 (AuNPs) 一起培养。通过CTAB-亚甲基蓝琼脂测定以及LC-MS/MS分析进一步分析鼠李糖脂的产生。根据 LC-MS/MS 结果，当与 CNP（1、5 和 10 mg/mL）和 AuNP（10、25、50 和 100 µg/mL）一起培养时，可以确认 K6 中对地鼠李糖脂的产生诱导。当 K6 培养物中添加 2.5 mg/mL CNP 和 10 µg/mL AuNP 时，观察到 K6 的单鼠李糖脂产量得到增强。还，在植物中的研究证实了鼠李糖脂的生物防治能力，因为它抑制了豇豆植物中的 Sclerotium rolfsii 感染。由于鼠李糖脂在农业领域具有广泛的应用，基于纳米的方法可以提高生物防治生物的产量。