In the present study, the doxorubicin-hydroxyapatite (DOX-HAp) has been loaded into the chitosan/PVA/PU single layer and core-shell nanofibers. The potential of synthesized nanofibers was evaluated for controlled release of DOX and bone cancer treatment in vitro. The synthesized DOX-HAp nanohybrid was characterized using XRD, SEM, and UV-Vis analysis. The morphology of synthesized nanofibers was examined by SEM and TEM analysis. For single layer nanofibers, some DOX-HAp nanohybrids were observed on the nanofibers surface. The fiber diameter was increased by increasing shell flow rate and no DOX-HAp nanohybrids were detected on the core-shell fibers surface. The DOX encapsulation efficiency of single layer and core-shell layer fibers was higher than 90 %. The initial burst release from single layer fiber at initial hours and the continuous release of DOX was observed from single layer nanofibers during 7 and 10 days under acidic and physiological pH. The sustained release of DOX was obtained within 10 and 14 days, 15 and 18 days, 21 and 25 days from core-shell fibers with flow rates of 0.3, 0.5 and 0.8 ml/h under acidic and physiological pH. The non-Fickian diffusion and Fickian diffusion were achieved from single layer and core-shell nanofibers. The cell attachment and cell death results indicated the high potential of DOX loaded-core-shell fibers for bone cancer treatment.

在本研究中，阿霉素-羟基磷灰石（DOX-HAp）已被装载到壳聚糖/ PVA / PU单层和核-壳纳米纤维中。评价了合成纳米纤维在DOX控释和体外骨癌治疗中的潜力。使用XRD，SEM和UV-Vis分析对合成的DOX-HAp纳米杂化物进行表征。通过SEM和TEM分析检查了合成的纳米纤维的形态。对于单层纳米纤维，在纳米纤维表面观察到一些DOX-HAp纳米杂化物。通过增加壳流速来增加纤维直径，并且在核-壳纤维表面上未检测到DOX-HAp纳米杂化物。单层和核-壳层纤维的DOX包封率均高于90％。在酸性和生理学pH下，在最初的几小时内，从单层纤维开始的突释释放和在单层纳米纤维的第7天和第10天观察到DOX的连续释放。在酸性和生理pH下，从壳壳纤维以0.3、0.5和0.8 ml / h的流速在10和14天，15和18天，21和25天之内获得了DOX的持续释放。从单层和核-壳纳米纤维获得非菲克扩散和菲克扩散。细胞附着和细胞死亡的结果表明DOX负载核壳纤维在骨癌治疗中具有很高的潜力。在酸性和生理pH值下，芯壳纤维的流动速率分别为0.3、0.5和0.8 ml / h的21天和25天。从单层和核-壳纳米纤维获得非菲克扩散和菲克扩散。细胞附着和细胞死亡的结果表明DOX负载核壳纤维在骨癌治疗中具有很高的潜力。在酸性和生理pH值下，芯壳纤维的流动速率分别为0.3、0.5和0.8 ml / h的21天和25天。从单层和核-壳纳米纤维获得非菲克扩散和菲克扩散。细胞附着和细胞死亡的结果表明DOX负载核壳纤维在骨癌治疗中具有很高的潜力。