In the present work, the biodegradable nanofibrous scaffolds containing poly(caprolactone) (PCL), poly(vinyl alcohol) (PVA) and aloe vera (AV), through two nozzles electrospinning method, were prepared and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement and mechanical analysis. The biocompatibility and cell growth were evaluated using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Adding poly(vinyl alcohol) (PVA) and poly(caprolactone) (PCL) boosted the electrospinability of aloe vera (AV) solution and mechanical features of the scaffolds. According to the Scanning electron microscopy (SEM) results, uniform PVA/AV-PCL nanofibers were fabricated with the average diameter about 119±11.78 nm. Fourier-transform infrared spectroscopy) FTIR (confirmed the presence of functional groups of scaffolds. The results of the contact angle showed that by adding aloe vera, the hydrophilicity increased. The results showed that the tensile strength of samples with 6 mg/ml AV, reached to 2.02 MPa. MTT results showed that all samples had a cell viability of over 80 %. It can be concluded that nanofibrous scaffolds with concentration of 6 mg/ml, distance between syringe tip/collector of 15 cm, feed rate of 3 ml/h and applied voltage of 12 kV, have appropriate properties for skin tissue engineering by stimulating the fibroblast cells and therefore accelerating the regeneration processes of damaged skin.

在本工作中，通过两个喷嘴电纺丝法制备了包含聚己内酯（PCL），聚乙烯醇（PVA）和芦荟（AV）的可生物降解的纳米纤维支架，并通过扫描电子显微镜，傅里叶变换对其进行了表征。红外光谱，接触角测量和机械分析。使用MTT（3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物）分析评估生物相容性和细胞生长。加入聚乙烯醇（PVA）和聚己内酯（PCL）可以提高芦荟（AV）溶液的电纺丝能力和支架的机械性能。根据扫描电子显微镜（SEM）的结果，制备出平均直径约为119±11.78nm的均匀PVA / AV-PCL纳米纤维。l AV，达到2.02 MPa。MTT结果表明，所有样品的细胞活力均超过80％。可以得出结论，浓度为6 mg / ml，注射器针尖/收集器之间的距离为15 cm，进料速度为3 ml / h和施加电压为12 kV的纳米纤维支架，具有通过刺激成纤维细胞进行皮肤组织工程改造的适当特性。细胞并因此加速受损皮肤的再生过程。