Abstract

Under the circumstance of wetland degradation, we used Biolog EcoPlates^TM method to investigate the impact of ecological restoration on the function of topsoil microbial communities by monitoring their metabolic diversity around Chaohu lakeside wetland. Four restoration patterns were selected, including reed shoaly land (RL), poplar plantation land (PL), abandoned shoaly grassland (GL) and cultivated flower land (FL). The result showed a rapid growth trend at the initial stage of incubation, following the fastest change rate at 72 h in both dormant and growing seasons. The Average Well Color development (AWCD) values of RL pattern was the highest at the detection points of each culture time, while the GL were the lowest. The calculation of diversity indicators also displayed significant lower McIntosh index in dormant season and Shannon-Wiener index in growing season in GL than in the others (P < 0.05). Carbohydrates and carboxylic acids were found to be the main substrates used in dormant season, whereas amino acids, polymers and phenolic acids were increasingly utilized by the microbial communities in growing season. We observed soil total potassium as the key factor that significantly affected the utilization efficiency of different carbon sources in both seasons (P < 0.05).

中文翻译：

---

使用Biolog EcoPlates™监测巢湖湖滨湿地的具有社区一级生理特征的土壤微生物

摘要

在湿地退化的情况下，我们使用了Biolog EcoPlates ^TM方法通过监测巢湖湖滨湿地的代谢多样性来研究生态恢复对表土微生物群落功能的影响。选择了四种恢复模式，包括芦苇浅滩地（RL），杨树人工林地（PL），废弃的浅滩草地（GL）和栽培花地（FL）。结果表明，在孵化初期，处于休眠和生长季节72 h最快的变化速率后，生长趋势迅速。在每个培养时间的检测点，RL模式的平均孔显色（AWCD）值最高，而GL最低。GL的多样性指标的计算还显示，与其他季节相比，GL的休眠季节的McIntosh指数和生长期的Shannon-Wiener指数显着较低（P<0.05）。发现碳水化合物和羧酸是休眠季节的主要底物，而氨基酸，聚合物和酚酸在生长季节被微生物群落越来越多地利用。我们观察到土壤总钾是在两个季节中显着影响不同碳源利用效率的关键因素（P <0.05）。