Heat shock protein 60 (HSP60) is a well-recognized multifunctional protein, playing a substantial role in protecting organisms from environmental stress. The domestic pigeon (Columba livia) is a promising model organism, with important economic and ecological value, and its health is susceptible to temperature stress. To explore the molecular characteristics, tissue expression profile, and response to temperature stress for HSP60 of Columba livia (ClHSP60), we firstly cloned and characterized the complete cDNA sequence and investigated its expression profile under optimal conditions and acute temperature stress. The cDNA of ClHSP60 contained 2257 nucleotides, consisting of 12 exons with length ranging from 65 to 590 bp. The open reading frame (ORF) encoded 573 amino acids with calculated molecular weight of 60.97 kDa that contained a number of structurally prominent domains or motifs. Under optimal temperature conditions, levels of ClHSP60 expression differed between all the tested tissues (the highest was noted in liver and the lowest in pectoralis major muscle). Under acute temperature stress, five patterns of change were detected in the tested tissues, suggesting that different tissues in domestic pigeons differentially responded to various temperature stress conditions. Upregulation of ClHSP60 expression was highest in the lung and pectoralis major muscle, reflecting the crucial role of these two tissues in temperature regulation. However, the crop, cerebrum, and heart showed little change or decreased ClHSP60 expression. The results indicate that ClHSP60 may be sensitive to and play pivotal roles in responding to acute temperature stress.

热休克蛋白 60 (HSP60) 是一种公认​​的多功能蛋白，在保护生物体免受环境压力方面发挥着重要作用。家鸽( Columba livia )是一种很有前景的模式生物，具有重要的经济和生态价值，其健康状况易受温度胁迫的影响。为了探究鸽子HSP60 （ Cl HSP60）的分子特征、组织表达谱和对温度胁迫的响应，我们首先克隆并表征了完整的cDNA序列，并研究了其在最佳条件和急性温度胁迫下的表达谱。 C1 HSP60的cDNA含有2257个核苷酸，由12个外显子组成，长度范围为65至590 bp。开放阅读框 (ORF) 编码 573 个氨基酸，计算分子量为 60.97 kDa，包含许多结构上突出的结构域或基序。在最佳温度条件下，所有测试组织之间的Cl HSP60 表达水平有所不同（肝脏中最高，胸大肌中最低）。在急性温度应激下，在测试的组织中检测到五种变化模式，表明家鸽的不同组织对不同温度应激条件的反应存在差异。 Cl HSP60 表达的上调在肺和胸大肌中最高，反映了这两种组织在温度调节中的关键作用。然而，嗉囊、大脑和心脏的Cl HSP60 表达几乎没有变化或降低。结果表明， Cl HSP60 可能对急性温度胁迫敏感，并在响应急性温度胁迫中发挥关键作用。