Colorimetric detection of creatinine using its specific binding peptides and gold nanoparticles,New Journal of Chemistry

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Colorimetric detection of creatinine using its specific binding peptides and gold nanoparticles
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020-09-02 , DOI: 10.1039/d0nj03860a
Shuaihui Feng _{1,

2,

3,

4,

5} , Rongjia Shi _{1,

2,

3,

4,

5} , Ping Xu _{1,

2,

3,

4,

5} , Jigna R. Bhamore _{1,

2,

3,

4,

5} , Jyotiranjan Bal _{1,

2,

3,

4,

5} , Seung Hoon Baek _{1,

2,

3,

4,

5} , Chan Yeong Park _{1,

2,

3,

4,

5} , Jong Pil Park _{3,

5,

6,

7,

8} , Tae Jung Park _{1,

2,

3,

4,

5}

Affiliation

Creatinine in serum and urine is an established biochemical marker for monitoring chronic kidney disease. Moreover, peptides possess unique attributes, such as a low molecular weight and the ease of synthesis and modification, which render them suitable for the recognition of creatinine. In this study, we obtained three kinds of peptides with specific affinities to creatinine by using the phage display technology. Further, a new method was developed for the selective detection of creatinine by combining gold nanoparticles (GNPs) and peptide probes; consequently, a creatinine complex was formed. This novel assay is based on the distance-dependent optical properties of GNPs. Due to the aggregation or dissociation of GNPs, the concentration of creatinine can be detected by visually monitoring the purple-red changes or using a UV-visible spectrometer. The proposed method was successfully employed for analyzing the creatinine content in human urine. The unique synergistic coordination between GNPs and peptides improves the selectivity and specificity for the creatinine detection. Furthermore, we believe that the proposed method can be utilized for the colorimetric detection of other biomolecules.

中文翻译：

肌酐的特异性结合肽和金纳米粒子比色检测

血清和尿液中的肌酐是监测慢性肾脏疾病的公认生化指标。此外，肽具有独特的属性，例如低分子量以及易于合成和修饰，这使其适合于肌酸酐的识别。在这项研究中，我们通过使用噬菌体展示技术获得了三种与肌酐具有特定亲和力的肽。此外，通过结合金纳米颗粒（GNP）和肽探针，开发了一种选择性检测肌酐的新方法。因此，形成了肌酸酐复合物。这种新颖的测定是基于GNP的距离依赖性光学特性。由于GNP的聚集或解离，可以通过肉眼监测紫红色变化或使用紫外可见光谱仪来检测肌酐的浓度。该方法成功地用于分析人尿中的肌酐含量。GNP和肽之间独特的协同配位可提高肌酐检测的选择性和特异性。此外，我们相信所提出的方法可以用于其他生物分子的比色检测。

更新日期：2020-09-28

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