Abstract

The biomaterials composed of mammalian extracellular matrix (ECM) have a great potential in pelvic floor tissue repair and functional reconstruction. However, bacterial infection does cause great damage to the repair function of biomaterials which is the major problem in clinical utilization. Therefore, the development of biological materials with antimicrobial effect is of great clinical significance for pelvic floor repair. Chitosan/tigecycline (CS/TGC) antibacterial biofilm was prepared by coating CS/TGC nanoparticles on mammalian-derived ECM. Infrared spectroscopy, scanning electron microscopy, bacteriostasis circle assay and static dialysis methods were used to characterize the membrane. MTS assay kit and DAPI fluorescence staining were used to evaluate cytotoxicity and cell adhesion. The biocompatibility was assessed by subabdominal implantation model in goats. Subcutaneous antimicrobial test in rabbit back was used to evaluate the antimicrobial and repairing effects on the infected wounds in vivo. Infrared spectroscopy showed that the composite coating had been successfully modified. The antibacterial membrane retained the main structure of ECM multilayer fibers. In vitro release of biomaterials showed sustained release and stability. In vivo studies showed that the antibacterial biological membrane had low cytotoxicity, fast degradation, good compatibility, anti-infection and excellent repair ability.

中文翻译：

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壳聚糖和替加环素涂覆的新型盆底修复抗菌生物材料网及其生物学性能

摘要

由哺乳动物细胞外基质（ECM）组成的生物材料在盆底组织修复和功能重建方面具有巨大潜力。然而细菌感染确实对生物材料的修复功能造成很大损害，是临床应用的主要问题。因此，开发具有抗菌作用的生物材料对于盆底修复具有重要的临床意义。壳聚糖/替加环素 (CS/TGC) 抗菌生物膜是通过在哺乳动物源性 ECM 上涂覆 CS/TGC 纳米颗粒而制备的。采用红外光谱、扫描电镜、抑菌圈测定和静态透析方法对膜进行表征。MTS测定试剂盒和DAPI荧光染色用于评估细胞毒性和细胞粘附。通过山羊腹下植入模型评估生物相容性。采用兔背部皮下抗菌试验，评价其对体内感染创面的抗菌及修复效果。红外光谱表明复合涂层已成功改性。抗菌膜保留了ECM多层纤维的主要结构。生物材料的体外释放表现出缓释性和稳定性。体内研究表明，该抗菌生物膜具有细胞毒性低、降解快、相容性好、抗感染和优异的修复能力。