Abstract A simple, rapid, sensitive, cheap and accurate oxidative method for two beta-blockers in pharmaceutical dosage forms was developed and evaluated. The method involved the oxidimetric treatment of atenolol and propanolol with 2 x 10-3 mol L-1 KMnO4 in alkaline medium (pH ≥ 9). Scanned spectra of oxidized complex showed distinctive absorptions at 460, 520, 540 and 570 nm. Arrays of colour changes were observed - from violet to blue; blue to bluish-green and yellow. Exhibited colours were due to ligand-metal charge transfer. An indirect spectrophotometric determination of atenolol and propranolol was done after 12-15 minutes at 520 nm. The optimum assay conditions showed linearity ranged from 0 – 15.0 μg mL-1 for both beta-blockers (R= 0.9997 – 0.9999). Molar absorptivity values were 4.79 x 103 and 4.88 x 103 L mol-1 cm-1 for atenolol and propanolol respectively, with corresponding Sandell’s sensitivity values of 0.056 and 0.053 μg cm-2. Limits of detection and quantification were 0.50 and 1.65 μg mL-1 for atenolol respectively and 0.58 and 1.91 μg mL-1 for propanolol, while relative standard deviation for intra-and inter-day precision were < 2.0%. The applicability, accuracy and reliability of the method were demonstrated by the determination of atenolol and propanolol in tablet formulations. The recovery studies ranged from 93.33 - 103.00% for both beta-blockers and the amounts in brands were from 97.53 ± 2.68 to 100.84 ± 1.82%.

中文翻译：

---

在碱性介质中使用高锰酸钾通过氧化衍生法测定两种 β 受体阻滞剂——阿替洛尔和普萘洛尔的光谱

摘要 开发并评估了一种简单、快速、灵敏、廉价且准确的药物剂型中两种β-受体阻滞剂的氧化方法。该方法包括在碱性介质 (pH ≥ 9) 中用 2 x 10-3 mol L-1 KMnO4 氧化处理阿替洛尔和心得安。氧化复合物的扫描光谱在 460、520、540 和 570 nm 处显示出独特的吸收。观察到一系列颜色变化——从紫色到蓝色；蓝色到蓝绿色和黄色。显示的颜色是由于配体-金属电荷转移。12-15 分钟后，在 520 nm 处对阿替洛尔和普萘洛尔进行间接分光光度法测定。两种 β 受体阻滞剂的最佳检测条件显示线性范围为 0 – 15.0 μg mL-1 (R= 0.9997 – 0.9999)。摩尔吸光度值为 4.79 x 103 和 4。阿替洛尔和心得安分别为 88 x 103 L mol-1 cm-1，相应的 Sandell 灵敏度值为 0.056 和 0.053 μg cm-2。阿替洛尔的检测限和定量限分别为 0.50 和 1.65 μg mL-1，心得安分别为 0.58 和 1.91 μg mL-1，而日内和日间精密度的相对标准偏差 < 2.0%。通过测定片剂配方中的阿替洛尔和普萘洛尔，证明了该方法的适用性、准确性和可靠性。两种 β 受体阻滞剂的回收率研究范围为 93.33 - 103.00%，品牌中的回收率范围为 97.53 ± 2.68 至 100.84 ± 1.82%。心得安为 91 μg mL-1，而日内和日间精密度的相对标准偏差 < 2.0%。通过测定片剂配方中的阿替洛尔和普萘洛尔，证明了该方法的适用性、准确性和可靠性。两种 β 受体阻滞剂的回收率研究范围为 93.33 - 103.00%，品牌中的回收率范围为 97.53 ± 2.68 至 100.84 ± 1.82%。心得安为 91 μg mL-1，而日内和日间精密度的相对标准偏差 < 2.0%。通过测定片剂配方中的阿替洛尔和普萘洛尔，证明了该方法的适用性、准确性和可靠性。两种 β 受体阻滞剂的回收率研究范围为 93.33 - 103.00%，品牌中的回收率范围为 97.53 ± 2.68 至 100.84 ± 1.82%。