ABSTRACT

1. To investigate the physiological role of FABP4 in the goose ovary, this study determined the effects of overexpressing and siRNA interfering FABP4 on progesterone (P₄) and oestradiol (E₂) production in granulosa cells. Measurements were made by ELISA, real-time qRT-PCR and western blotting.

2. The concentrations of P₄ and E₂ in the FABP4 overexpression granulosa cells were increased compared to the control group (P > 0.05 for P₄; P < 0.05 for E₂). Likewise, the mRNA and protein expression levels of CYP11A1 and CYP19A1 were significantly higher than in the control group (P < 0.05 or P < 0.001). Conversely, the concentrations of P₄ and E₂ in the FABP4 silencing granulosa cells were significantly decreased compared with the control group (P < 0.001). Likewise, the mRNA and protein expression levels of CYP11A1 and CYP19A1 were significantly lower than in the control group (P < 0.001, or P < 0.01).

3. The study indicated that the FABP4 gene may regulate steroid hormone secretion and the expression of the steroidogenic genes in geese ovarian granulosa cells. These results support the possibility that the FABP4 gene mediates ovarian steroid hormone biosynthesis function and reproduction in geese.

摘要

1.为了研究FABP4在鹅卵巢中的生理作用，本研究确定了过表达和siRNA干扰FABP4对颗粒细胞中孕酮（P ₄）和雌二醇（E ₂）的产生。通过ELISA，实时qRT-PCR和蛋白质印迹进行测量。

2. P的浓度₄和E ₂中的FABP4相比于对照组过表达颗粒细胞均增加（P> 0.05对于P ₄ ; P <0.05对于E ₂）。同样，CYP11A1和CYP19A1的mRNA和蛋白表达水平也明显高于对照组（P <0.05或P <0.001）。相反，与对照组相比，FABP4沉默颗粒细胞中P ₄和E ₂的浓度显着降低（P <0.001）。同样地，CYP11A1和CYP19A1的mRNA和蛋白质表达水平 明显低于对照组（P <0.001，或P <0.01）。

3.研究表明FABP4基因可能调节鹅卵巢颗粒细胞中类固醇激素的分泌和类固醇生成基因的表达。这些结果支持FABP4基因介导鹅中卵巢类固醇激素生物合成功能和繁殖的可能性。