The uptake of specific fatty acids in humans is dependent on their position on the glycerol backbone. There is a great interest in methods that can access this information fast and accurately. By way of high‐resolution NMR, we have analyzed TAG extracted from pig and beef tissues and obtained quantitative data for the composition and regioisomeric distribution of all major unsaturated fatty acids usually found in these source materials, using a combination of manual integration and deconvolution of ¹³C NMR spectra. In addition, we have developed a method for determining composition and regioisomeric distribution of the two main saturated fatty acids found in pork (16:0, 18:0). The results are discussed in relation to species‐specific genetic characteristics of fatty acid and TAG biosynthesis. The developed method could support decisions related to breeding for desired fatty acid profiles, and stimulate further methodology developments using high field NMR.

中文翻译：

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使用 13 C NMR 光谱法定量分析猪和牛来源的三酰甘油中的脂肪酸及其区域异构分布。

人体对特定脂肪酸的摄取取决于它们在甘油骨架上的位置。人们对可以快速准确地访问这些信息的方法非常感兴趣。通过高分辨率 NMR，我们分析了从猪和牛肉组织中提取的 TAG 并获得了这些源材料中常见的所有主要不饱和脂肪酸的组成和区域异构分布的定量数据，使用手动积分和解卷积的组合¹³C核磁共振谱。此外，我们还开发了一种确定猪肉中发现的两种主要饱和脂肪酸的组成和区域异构分布的方法 (16:0, 18:0)。讨论了与脂肪酸和 TAG 生物合成的物种特异性遗传特征相关的结果。开发的方法可以支持与所需脂肪酸谱育种相关的决策，并使用高场 NMR 刺激进一步的方法开发。