N4‐methylcytidine ribosomal RNA methylation in chloroplasts is crucial for chloroplast function, development, and abscisic acid response in Arabidopsis,Journal of Integrative Plant Biology

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N4‐methylcytidine ribosomal RNA methylation in chloroplasts is crucial for chloroplast function, development, and abscisic acid response in Arabidopsis
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-09-02 , DOI: 10.1111/jipb.13009
Le Nguyen Tieu Ngoc _{1,

2} , Su Jung Park ₁ , Trinh Thi Huong ₁ , Kwang Ho Lee ₃ , Hunseung Kang ₁

Affiliation

Although the essential role of messenger RNA methylation in the nucleus is increasingly understood, the nature of ribosomal RNA (rRNA) methyltransferases and the role of rRNA methylation in chloroplasts remain largely unknown. A recent study revealed that CMAL (for Chloroplast mr aW‐ Like) is a chloroplast‐localized rRNA methyltransferase that is responsible for N4‐methylcytidine (m⁴C) in 16S chloroplast rRNA in Arabidopsis thaliana. In this study, we further examined the role of CMAL in chloroplast biogenesis and function, development, and hormone response. The cmal mutant showed reduced chlorophyll biosynthesis, photosynthetic activity, and growth‐defect phenotypes, including severely stunted stems, fewer siliques, and lower seed yield. The cmal mutant was hypersensitive to chloroplast translation inhibitors, such as lincomycin and erythromycin, indicating that the m⁴C‐methylation defect in the 16S rRNA leads to a reduced translational activity in chloroplasts. Importantly, the stunted stem of the cmal mutant was partially rescued by exogenous gibberellic acid or auxin. The cmal mutant grew poorer than wild type, whereas the CMAL‐overexpressing transgenic Arabidopsis plants grew better than wild type in the presence of abscisic acid. Altogether, these results indicate that CMAL is an indispensable rRNA methyltransferase in chloroplasts and is crucial for chloroplast biogenesis and function, photosynthesis, and hormone response during plant growth and development.

中文翻译：

叶绿体中的N4-甲基胞苷核糖体RNA甲基化对于拟南芥中的叶绿体功能，发育和脱落酸应答至关重要

尽管信使RNA甲基化在细胞核中的重要作用已得到越来越多的了解，但核糖体RNA（rRNA）甲基转移酶的性质和叶绿体中rRNA甲基化的作用仍然未知。最近的一项研究揭示，CMAL（对于ç hloroplast米ř 一个W- 大号IKE）是叶绿体定位的的rRNA甲基转移酶，其负责N4甲基胞苷（M ⁴中C）在16S rRNA基因叶绿体拟南芥。在这项研究中，我们进一步检查了CMAL在叶绿体生物发生，功能，发育和激素反应中的作用。该CMAL该突变体显示出叶绿素的生物合成减少，光合作用活性和生长缺陷表型，包括茎严重发育不良，长角果较少和种子产量降低。所述CMAL突变体过敏的叶绿体翻译抑制剂，例如林可霉素和红霉素，表明米⁴中的16S rRNA导致降低的翻译活性叶绿体C-甲基化缺陷。重要的是，外源赤霉素或生长素可部分拯救cmal突变体的发育不良茎。该CMAL突变体增长速度比野生型较差，而CMAL过表达转基因拟南芥在脱落酸的存在下，植物的生长优于野生型。总之，这些结果表明，CMAL是叶绿体中必不可少的rRNA甲基转移酶，对于植物生长和发育过程中的叶绿体生物发生，功能，光合作用和激素反应至关重要。

更新日期：2020-09-02

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