The Arabidopsis phosphatase PP2C49 negatively regulates salt tolerance through inhibition of AtHKT1;1,Journal of Integrative Plant Biology

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The Arabidopsis phosphatase PP2C49 negatively regulates salt tolerance through inhibition of AtHKT1;1
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-09-02 , DOI: 10.1111/jipb.13008
Moli Chu ₁ , Pengwang Chen ₁ , Sufang Meng ₁ , Peng Xu ₁ , Wenzhi Lan ₁

Affiliation

Type 2C protein phosphatases (PP2Cs) are the largest protein phosphatase family. PP2Cs dephosphorylate substrates for signaling in Arabidopsis, but the functions of most PP2Cs remain unknown. Here, we characterized PP2C49 (AT3G62260, a Group G PP2C), which regulates Na⁺ distribution under salt stress and is localized to the cytoplasm and nucleus. PP2C49 was highly expressed in root vascular tissues and its disruption enhanced plant tolerance to salt stress. Compared with wild type, the pp2c49 mutant contained more Na⁺ in roots but less Na⁺ in shoots and xylem sap, suggesting that PP2C49 regulates shoot Na⁺ extrusion. Reciprocal grafting revealed a root‐based mechanism underlying the salt tolerance of pp2c49. Systemic Na⁺ distribution largely depends on AtHKT1;1 and loss of function of AtHKT1;1 in the pp2c49 background overrode the salt tolerance of pp2c49, resulting in salt sensitivity. Furthermore, compared with plants overexpressing PP2C49 in the wild‐type background, plants overexpressing PP2C49 in the athtk1;1 mutant background were sensitive to salt, like the athtk1;1 mutants. Moreover, protein–protein interaction and two‐voltage clamping assays demonstrated that PP2C49 physically interacts with AtHKT1;1 and inhibits the Na⁺ permeability of AtHKT1;1. This study reveals that PP2C49 negatively regulates AtHKT1;1 activity and thus determines systemic Na⁺ allocation during salt stress.

中文翻译：

拟南芥磷酸酶 PP2C49 通过抑制 AtHKT1 负向调节耐盐性；1

2C 型蛋白磷酸酶 (PP2C) 是最大的蛋白磷酸酶家族。PP2Cs 使拟南芥中信号的底物去磷酸化，但大多数 PP2Cs 的功能仍然未知。在这里，我们表征了 PP2C49（AT3G62260，G 组 PP2C），它在盐胁迫下调节 Na ⁺分布并定位于细胞质和细胞核。PP2C49在根维管组织中高表达，其破坏增强了植物对盐胁迫的耐受性。与野生型相比，pp2c49突变体根部Na ⁺含量较高，但地上部和木质部汁液中Na ⁺含量较少，表明PP2C49对地上部Na ^{+ 有}调节作用。挤压。相互嫁接揭示了pp2c49耐盐性的基于根的机制。全身的Na ⁺分布在很大程度上取决于AtHKT1; 1和功能丧失1; AtHKT1在pp2c49背景压倒的耐盐性pp2c49，从而导致盐的敏感性。此外，与植物相比过表达PP2C49在野生型背景，植物过量表达PP2C49在athtk1; 1个突变体背景是对盐敏感，像athtk1; 1突变体。此外，蛋白质-蛋白质相互作用和双电压钳夹试验表明 PP2C49 与 AtHKT1;1 物理相互作用并抑制 AtHKT1;1 的 Na ⁺渗透性。该研究表明，PP2C49 负向调节 AtHKT1;1 活性，从而决定盐胁迫期间系统 Na ^{+ 的}分配。

更新日期：2020-09-02

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