Crystal structure of bacteriophage T4 Spackle as determined by native SAD phasing.,Acta Crystallographica Section D

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Crystal structure of bacteriophage T4 Spackle as determined by native SAD phasing.
Acta Crystallographica Section D ( IF 2.6 ) Pub Date : 2020-09-02 , DOI: 10.1107/s2059798320010979
Ke Shi ₁ , Fredy Kurniawan ₁ , Surajit Banerjee ₂ , Nicholas H Moeller ₁ , Hideki Aihara ₁

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The crystal structure of a bacteriophage T4 early gene product, Spackle, was determined by native sulfur single‐wavelength anomalous diffraction (SAD) phasing using synchrotron radiation and was refined to 1.52 Å resolution. The structure shows that Spackle consists of a bundle of five α‐helices, forming a relatively flat disc‐like overall shape. Although Spackle forms a dimer in the crystal, size‐exclusion chromatography with multi‐angle light scattering shows that it is monomeric in solution. Mass spectrometry confirms that purified mature Spackle lacks the amino‐terminal signal peptide and contains an intramolecular disulfide bond, consistent with its proposed role in the periplasm of T4 phage‐infected Escherichia coli cells. The surface electrostatic potential of Spackle shows a strikingly bipolar charge distribution, suggesting a possible mode of membrane association and inhibition of the tail lysozyme activity in T4 bacteriophage superinfection exclusion.

中文翻译：

通过天然 SAD 定相确定的噬菌体 T4 Spackle 的晶体结构。

噬菌体 T4 早期基因产物 Spackle 的晶体结构是通过使用同步辐射的天然硫单波长异常衍射 (SAD) 定相来确定的，并精炼至 1.52 Å 分辨率。结构表明，Spackle由一束五个α螺旋组成，形成相对平坦的盘状整体形状。尽管 Spackle 在晶体中形成二聚体，但具有多角度光散射的尺寸排阻色谱表明它在溶液中是单体。质谱分析证实纯化的成熟 Spackle 缺乏氨基末端信号肽并含有分子内二硫键，这与其在 T4 噬菌体感染的大肠杆菌细胞周质中的作用一致。 Spackle 的表面静电势显示出惊人的双极电荷分布，表明在 T4 噬菌体重复感染排除中膜缔合和尾部溶菌酶活性抑制的可能模式。

更新日期：2020-09-02

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