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Molecular correlates of MRS-based 31 phosphocreatine muscle resynthesis rate in healthy adults.
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2020-09-01 , DOI: 10.1002/nbm.4402
Moses M Darpolor 1 , Maninder Singh 1 , Jeffrey Covington 1 , Sebastian Hanet 1 , Eric Ravussin 1 , Owen T Carmichael 1
Affiliation  

Dynamic phosphorus MRS (31P‐MRS) is a method used for in vivo studies of skeletal muscle energetics including measurements of phosphocreatine (PCr) resynthesis rate during recovery of submaximal exercise. However, the molecular events associated with the PCr resynthesis rate are still under debate. We assessed vastus lateralis PCr resynthesis rate from 31P‐MRS spectra collected from healthy adults as part of the CALERIE II study (caloric restriction), and assessed associations between PCr resynthesis and muscle mitochondrial signature transcripts and proteins (NAMPT, NQO1, PGC‐1α, and SIRT1). Regression analysis indicated that higher concentration of nicotinamide phosphoribosyltransferase (NAMPT) protein, a mitochondrial capacity marker, was associated with faster PCr resynthesis. However, PCr resynthesis was not associated with greater physical fitness (VO2 peak) or messenger ribonucleic acid levels of mitochondrial function markers such as NQO1, PGC‐1α, and SIRT1, suggesting that the impact of these molecular signatures on PCr resynthesis may be minimal in the context of an acute exercise bout. Together, these findings suggest that 31P‐MRS based PCr resynthesis may represent a valid non‐invasive surrogate marker of mitochondrial NAMPT in human skeletal muscle.

中文翻译:

健康成人中基于 MRS 的 31 磷酸肌酸肌肉再合成率的分子相关性。

动态磷 MRS ( 31 P-MRS) 是一种用于骨骼肌能量学的体内研究的方法,包括在次最大运动恢复期间测量磷酸肌酸 (PCr) 再合成率。然而,与 PCr 再合成速率相关的分子事件仍在争论中。我们从31评估了股外侧肌 PCr 再合成率作为 CALERIE II 研究(热量限制)的一部分,从健康成人收集的 P-MRS 光谱,并评估了 PCr 再合成与肌肉线粒体特征转录物和蛋白质(NAMPT、NQO1、PGC-1α 和 SIRT1)之间的关联。回归分析表明,较高浓度的烟酰胺磷酸核糖基转移酶 (NAMPT) 蛋白(一种线粒体容量标志物)与更快的 PCr 再合成相关。然而,PCr 再合成与更高的体能(VO 2峰值)或线粒体功能标志物(如 NQO1、PGC-1α 和 SIRT1)的信使核糖核酸水平无关,这表明这些分子特征对 PCr 再合成的影响可能很小在剧烈运动的情况下。总之,这些发现表明31基于 P-MRS 的 PCr 再合成可能代表人类骨骼肌中线粒体 NAMPT 的有效非侵入性替代标记。
更新日期:2020-09-01
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