A potential entanglement between the spinal cord and hippocampus: Theta rhythm correlates with neurogenesis deficiency following spinal cord injury in male rats.,Journal of Neuroscience Research

当前位置： X-MOL 学术 › J. Neurosci. Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A potential entanglement between the spinal cord and hippocampus: Theta rhythm correlates with neurogenesis deficiency following spinal cord injury in male rats.
Journal of Neuroscience Research ( IF 2.9 ) Pub Date : 2020-09-01 , DOI: 10.1002/jnr.24719
Hamid Soltani Zangbar _{1,

2,

3} , Tahereh Ghadiri ₁ , Manuchehr Seyedi Vafaee ₄ , Abbas Ebrahimi Kalan ₁ , Mohammad Karimipour ₅ , Solmaz Fallahi ₆ , Meysam Ghorbani ₆ , Parviz Shahabi _{2,

3}

Affiliation

Cognitive deficits due to spinal cord injury (SCI) have been elucidated in both animals and humans with SCI. Such disorders may cause concomitant oscillatory changes in regions of the brain involving in cognition; a subject that has not been directed mechanistically. One of the crucial oscillations, having a prominent role in cognition, particularly spatial memory, is hippocampal theta rhythm. Our research revealed that SCI could induce changes not only in the neurogenesis and apoptosis rate of the hippocampus but also in theta power as well as receptors involving in the generation of this rhythm. Herein we used 24 male Wistar rats (Sham/SCI = 12) and examined the effect of spinal cord contusion on hippocampal theta rhythm, spatial memory, and neurodegeneration. We proved that SCI eliminates hippocampus‐dependent theta power through spatial working memory, and correlates significantly with neurodegeneration and expression of receptors (NMDA, GABAA, Muscarinic1/M1), which are in turn essential in generation of theta rhythm. The immunohistochemistry analysis also demonstrated a significant decrease in DCX+ and BrdU+ cells; however, according to TUNEL assay, apoptosis is significantly higher in SCI‐induced animals. The western blotting analysis further showed a significant reduction of the abovementioned receptors in the hippocampus. We also verified that SCI impairs the spatial memory, proved by poor performance in the Y‐maze task. As well as, based on the local field potential recordings analysis, SCI decreases the power of theta rhythm. Eventually, this study demonstrated that chronic brain neurodegeneration occurs after SCI accompanied by theta rhythm and cognitive deficiency.

中文翻译：

脊髓和海马体之间的潜在纠缠：Theta 节律与雄性大鼠脊髓损伤后的神经发生缺陷相关。

脊髓损伤 (SCI) 导致的认知缺陷已在动物和 SCI 患者中得到阐明。此类障碍可能会导致大脑中涉及认知的区域伴随的振荡变化；一个没有被机械地指导的主题。在认知（尤其是空间记忆）中具有重要作用的关键振荡之一是海马 theta 节律。我们的研究表明，SCI 不仅可以引起海马神经发生和细胞凋亡率的变化，还可以引起θ功率以及参与这种节律产生的受体的变化。在此，我们使用了 24 只雄性 Wistar 大鼠（Sham/SCI = 12）并检查了脊髓挫伤对海马 theta 节律、空间记忆和神经变性的影响。我们证明 SCI 通过空间工作记忆消除海马依赖的 theta 能量，并与神经退行性变和受体（NMDA、GABAA、Muscarinic1/M1）的表达显着相关，而这些又对 theta 节律的产生至关重要。免疫组织化学分析还表明 DCX+ 和 BrdU+ 细胞显着减少；然而，根据 TUNEL 测定，SCI 诱导的动物的细胞凋亡显着更高。蛋白质印迹分析进一步显示海马中上述受体显着减少。我们还验证了 SCI 会损害空间记忆，这在 Y 迷宫任务中表现不佳就证明了这一点。此外，基于局部场电位记录分析，SCI 降低了 theta 节律的力量。最终，

更新日期：2020-10-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11