Biological pretreatment is feasible for enhancing methane generation with crop residues. Improvement of the degradation efficiency of cellulose has always been the key to improve methane yield. The role of an anaerobic and thermophilic bacterium, Caldicellulosiruptor sp. F32, on the degradation of corn stalk was investigated. A pure bacterial pretreatment system (0.20 v/v), with 20 times CFU of 2.20$\times$10⁸ was verified to be beneficial to the deterioration of corn stalk residues and the increase of methane output. The highest methane yield was 250.2 mL/g volatile solid, which was 28% higher than that from the untreated group. In group 4, the concentrations of glucose and volatile fatty acids were higher than those in other groups. Furthermore, the assays by Fourier-transform infrared spectra and X-ray diffraction revealed that the crystallinity indexes of pretreated groups declined. Hence, biology-based pretreatment using pure bacterial system, Caldicellulosiruptor sp. F32, is a potentially effective approach to improve the degradation efficiency of lignocellulosic biomass.

生物预处理对于提高农作物残留甲烷的产生是可行的。纤维素降解效率的提高一直是提高甲烷产量的关键。厌氧和嗜热细菌Caldicellulosiruptor sp。的作用。F32，对玉米秸秆的降解进行了研究。纯细菌预处理系统（0.20 v / v），20倍CFU为2.20$\times$证实10 ⁸对玉米秸秆残留物的恶化和甲烷产量的增加是有益的。最高甲烷产量为250.2 mL / g挥发性固体，比未处理组高28％。在第4组中，葡萄糖和挥发性脂肪酸的浓度高于其他组。此外，通过傅立叶变换红外光谱和X射线衍射的测定表明，预处理基团的结晶度指数下降。因此，使用纯细菌系统Caldicellulosiruptor sp。进行基于生物学的预处理。F32是提高木质纤维素生物质降解效率的潜在有效途径。