Increasing soil stabilization with traditional additives such as cement, which release a high quantity of greenhouse gases during the production, raises environmental concerns. Thus, as a promising alternative for replacing traditional additives, biological processes are emerging as desired green technologies for soil improvement. In this study, a procedure was investigated to improve soil inter-particle cohesion using an environmentally friendly microbial polysaccharide. Further, specific approaches were adopted to reduce the production costs including use of cheap substrates, along with minimizing downstream processing expenses. A novel strain of Pantoea which exhibited a good potential of biopolymer production was isolated from an iron mine. The identification and phylogenetic studies of the isolated strain based on 16S rRNA gen confirmed that the studied strain certainly belongs to Pantoea strain of Enterobacteriaceae. Unconfined compressive strength (UCS) tests confirmed the biopolymer’s potential for clay stabilization. The results indicated that the interaction between the non-extracted biopolymer in the form of cell-free broth (CFB) and soil particles enhanced soil stability. The greatest increase in the UCS was observed in 28 days of curing using 25% CFB/optimum moisture content (OMC), which was 21% more than the UCS value of the untreated soil.

使用传统添加剂（例如水泥）提高土壤稳定性，在生产过程中会释放大量温室气体，这引起了环境方面的关注。因此，作为替代传统添加剂的有前途的替代方法，生物过程正在作为土壤改良的绿色技术兴起。在这项研究中，研究了一种使用环境友好的微生物多糖改善土壤间颗粒内聚力的方法。此外，采用了特定的方法来降低生产成本，包括使用廉价的基板，并最大程度地减少下游处理费用。一种新型的泛菌从铁矿中分离出具有良好生物聚合物生产潜力的产品。根据16S rRNA基因分离菌株的鉴定和系统发育研究证实，该菌株确实属于肠杆菌科的泛菌菌株。无限制抗压强度（UCS）测试证实了该生物聚合物具有稳定粘土的潜力。结果表明，无细胞肉汤（CFB）形式的未提取生物聚合物与土壤颗粒之间的相互作用增强了土壤的稳定性。在使用28％的CFB /最佳含水量（OMC）固化28天后，观察到UCS的最大增加，这比未经处理的土壤的UCS值高21％。