Replacing sucrose with non-caloric sweeteners is an approach to avoid overweight and diabetes development. Non-caloric sweeteners are classified into either artificial as sucralose or natural as stevia. Both of them have been approved by FDA, but the effects of their chronic consumption are controversial. The present study aimed to evaluate the effects of these two sweeteners, in male and female albino mice, on different blood biochemical parameters, enzymes activities and immunological parameters after 8 and 16 weeks of sweeteners administration. 40.5 mg/ml of sucrose, 5.2 mg/ml of sucralose and 4.2 mg/ml of stevia were dissolved individually in distilled water. Mice were administrated by sweetener's solution for 5 h daily. Male and female mice showed a preference for water consumption with sucralose or stevia. Both of the two sweeteners significantly reduced the hemoglobin level, HCT%, RBCs and WBCs count. After 18 weeks, significant elevations in liver and kidney function enzymes were observed in male and female mice administrated with both non-caloric sweeteners. Histopathological examination in sucralose and stevia administrated groups confirmed the biochemical results; where it revealed a severe damage in liver and kidney sections. While, sucrose administration elevated, only, the levels of ALT, AST and cholesterol in male mice. A vigorous elevation in levels of different immunoglobulin (IgG, IgE and IgA) and pro-inflammatory cytokines (IL-6 and -8), that was accompanied by a significant reduction in level of anti-inflammatory cytokine IL-10, was observed in male and female mice groups administrated with sucralose or stevia. On the other hand, sucrose administration led to an elevation in IgA and reduction in IL-10 levels.

用非热量甜味剂代替蔗糖是避免超重和糖尿病发展的一种方法。非热量甜味剂分为三氯蔗糖的人造甜味剂或甜叶菊的天然甜味剂。两者均已获得FDA批准，但其长期食用的影响尚存争议。本研究旨在评估雄性和雌性白化病小鼠中这两种甜味剂对甜味剂施用8周和16周后不同血液生化参数，酶活性和免疫学参数的影响。将40.5 mg / ml的蔗糖，5.2 mg / ml的三氯蔗糖和4.2 mg / ml的甜菊糖分别溶解在蒸馏水中。每天通过甜味剂溶液对小鼠给药5小时。雄性和雌性小鼠对三氯蔗糖或甜叶菊的饮水表现出偏爱。两种甜味剂均显着降低了血红蛋白水平，HCT％，RBC和WBC计数。18周后，在使用两种非热量甜味剂的雄性和雌性小鼠中观察到肝和肾功能酶的显着升高。三氯蔗糖和甜菊给药组的组织病理学检查证实了其生化结果。那里显示出肝脏和肾脏部分严重受损。虽然蔗糖的施用仅使雄性小鼠的ALT，AST和胆固醇水平升高。观察到，不同免疫球蛋白（IgG，IgE和IgA）和促炎细胞因子（IL-6和-8）的水平急剧升高，同时消炎细胞因子IL-10的水平显着降低。用三氯蔗糖或甜叶菊给药的雄性和雌性小鼠组。另一方面，