The objective of this study was to characterize an endophytic fungi producing-bioactive compound from the aquatic plant, Nelumbo nucifera. All parts of such plant were cleaned with surface sterilization technique and cultured on potato dextrose agar to isolate endophytic fungi. The identification was characterized by morphological and molecular technique. Fungal isolates were screened to discover antimicrobial activities by disc diffusion method against Methicillin-resistant Staphylococcus aureus DMST20651 (MRSA). MIC and MBC for those crude fungal extracts were determined. Finally, the chemical profile of crude extract was determined by gas chromatography and mass spectrometry. Six endophytic fungi were isolated from the surface-satirized parts of N. nucifera. Based on disc diffusion assay, the highest antibacterial activity against MRSA was isolate ST9.1 identified as Aspergillus cejpii. Results demonstrated that the ethyl acetate extraction had more active fractions with MIC of 2.5 mg/ml and MBC concentration of 50.0 mg/ml. The crude extracts were developed to identify the chemical constituents by gas chromatography and mass spectrometry. The major component of crude extract of endophytic fungi was 5-(1H-Indol-3-yl)-4,5-dihydro-[1,2,4]triazin-3-ylamine (C₁₁H₁₁N₅). Thus, the plant could be used in the treatment of infectious diseases caused by bacterial pathogen.

这项研究的目的是鉴定水生植物Nelumbo nucifera的内生真菌产生生物活性化合物。用表面灭菌技术清洁该植物的所有部分，并在马铃薯葡萄糖琼脂上培养以分离内生真菌。鉴定通过形态学和分子技术表征。通过盘扩散法筛选真菌分离物以发现抗甲氧西林金黄色葡萄球菌DMST20651（MRSA）的抗菌活性。确定了那些粗真菌提取物的MIC和MBC。最后，通过气相色谱和质谱法确定粗提取物的化学特征。从N. nucifera的表面饱和的部分中分离出六种内生真菌。基于圆盘扩散测定法，对MRSA的最高抗菌活性是分离的ST9.1菌株，被鉴定为ceperii cejpii。结果表明，乙酸乙酯萃取的活性级分更高，MIC为2.5 mg / ml，MBC浓度为50.0 mg / ml。开发粗提物以通过气相色谱法和质谱法鉴定化学成分。内生真菌粗提物的主要成分是5-（1H-吲哚-3-基）-4,5-二氢-[1,2,4]三嗪-3-基胺（C ₁₁ H ₁₁ N ₅）。因此，该植物可用于治疗由细菌病原体引起的传染病。