A comparison of high-performance liquid chromatography (HPLC) and solid-phase extraction (SPE) methods is carried out for the simultaneous analysis of indoxyl sulfate and sodium butyrate in plasma using Phenyl (250 x 4.6 mm; 5 µm) and SunShell (150 x 4.6 mm, 2.6 µm) columns with mobile phases ammonium acetate (20 mM)-ACN (95:5, v/v) and water-ACN (90:10), respectively. The values of the retention, separation and resolution factors for indoxyl sulfate and sodium butyrate were 3.1, 1.29 and 2.0 with phenyl and 1.0, 1.55 and 1.60 with SunShell columns. The values of the theoretical plates were in the range of 650 to 1600 while the tailing factors were 0.98 to 1.0. The limits of the detection and quantification of sodium butyrate and indoxyl sulfate were 0.1 to 1.9 and 0.43 to 9.2 µg with these columns. The hydrogen bondings and π-π interactions were responsible for the separation of these molecules. The multi-wall carbon nanotubes (MWCNTs) served as a good sorbent in SPE extraction. The developed SPE and HPLC methods were quick, robust, exact and economic for the analysis of indoxyl sulfate and sodium butyrate. The reported SPE and HPLC methods can be useful to determine the reported molecules in plasma.

进行了高效液相色谱（HPLC）和固相萃取（SPE）方法的比较，以使用苯基（250 x 4.6 mm; 5 µm）和SunShell（150）同时分析血浆中的吲哚酚硫酸盐和丁酸钠x 4.6 mm，2.6 µm）色谱柱，分别用乙酸铵（20 mM）-ACN（95：5，v / v）和水-ACN（90:10）流动相。苯基吲哚酚硫酸盐和丁酸钠的保留，分离和分离度值分别为苯基，3.1、1.29和2.0，SunShell色谱柱分别为1.0、1.55和1.60。理论塔板的值在650至1600的范围内，而拖尾系数为0.98至1.0。用这些色谱柱对丁酸钠和吲哚酚硫酸盐的检测和定量限为0.1至1.9和0.43至9.2 µg。氢键和π-π相互作用负责这些分子的分离。多壁碳纳米管（MWCNT）在SPE萃取中起良好的吸附作用。所开发的SPE和HPLC方法快速，稳定，准确，经济，可用于分析吲哚基硫酸盐和丁酸钠。报道的SPE和HPLC方法可用于确定血浆中报道的分子。