Bambusa balcooa Roxb. is one of the economically most important bamboo species cultivated in the tropical countries. The present investigation is conceded to establish an effective method for clonal propagation of B. balcooa. The morphological response from nodal cuttings was tested on Murashige and Skoog’s (MS) basal medium mixed with a range of combinations and concentrations of 6-furfurylaminopurine (Kinetin) and 6-benzylaminopurine (BAP). About 96 % shoot sprouting was observed on medium containing 4.0 mg L⁻¹ BAP, 50 mg L⁻¹ ascorbic acid and 25 mg L⁻¹ each of l-arginine, citric acid and adenine sulphate. These shoots were proliferated efficiently on MS medium with 4.0 mg L⁻¹ BAP, 1.0 mg L⁻¹ NAA, which resulted in the formation of ∼62.0 shoots per node per culture bottle. In vitro rooting was achieved on ½-strength MS basal medium with 6.0 mg L⁻¹ NAA and 100 mg L⁻¹ activated charcoal. About one-month-old well-rooted plantlets were exposed to the greenhouse for 4 weeks in soilrite®. The hardened plantlets exhibited 100 % survival success under the field environment after 3 months. The monomorphic pattern of inter simple sequence repeats (ISSR) and start codon targeted (SCoT) markers of in vitro raised plants matched with mother plants confirmed the genetic stability. Moreover, in vitro raised plantlest also showed histological similarties with mother plant. Hence, this method could be efficiently utilized for commercial-scale production of B. balcooa.

Bambusa balcooa Roxb。是热带国家种植的经济上最重要的竹种之一。本研究承认建立一种有效的B. balcooa克隆繁殖方法。在Murashige和Skoog's（MS）基础培养基上混合了一系列6-6-糠基氨基嘌呤（激动素）和6-苄基氨基嘌呤（BAP）的浓度，测试了来自节点cutting插的形态响应。在含有4.0 mg L ^-1 BAP，50 mg L ^-1抗坏血酸和25 mg L ^-1的1-精氨酸，柠檬酸和腺嘌呤硫酸盐的培养基上观察到约96％的芽萌芽。这些芽在含有4.0 mg L ^-1的MS培养基上有效增殖BAP，1.0 mg L ^-1 NAA，导致每个培养瓶每个结点形成约62.0个芽。在具有6.0 mg L ^-1 NAA和100​​ mg L ^-1活性炭的1/2强度MS基础培养基上实现了生根。大约一个月大的根茎苗在土壤中暴露于温室中4周。硬化的小苗在田间环境下3个月后显示100％的存活成功。与母本植物匹配的体外培育植物间简单序列重复（ISSR）和起始密码子靶向（SCoT）标记的单态性证实了遗传稳定性。而且，体外种植的小植株也显示出与母株的组织学相似性。因此，该方法可以有效地用于商业规模的B. balcooa的生产。