A 56-day research was performed to examine the influence of graded levels (0 (control), 0.5, 1 and 2%) of Fern (Adiantum capillus-veneris) leaves powder (FLP) in diet on immune competence and growth of common carp (Cyprinus carpio, initial weight = 20 g). The serum total immunoglobulins content and lysozyme activity in the 1 and 2% FLP groups remarkably increased compared to the other groups (P < 0.05). The skin mucosal lysozyme activity enhanced with increasing dietary FLP level in a dose-response manner. Fish fed on the FLP-supplemented diets had higher skin-mucosal superoxide dismutase activity than the control (P < 0.05). However, serum antioxidant enzymes were not affected by dietary fern (P > 0.05). The serum bactericidal activity against human and fish pathogens increased with enhancing the FLP level in diet against Staphylococcus aureus, Escherichia coli (EHEC ATCC 43895), Escherichia coli (CI), Pseudomonas aeruginosa, Klebsiella pneumonia and Aeromonas hydrophila. The serum antibacterial activity against Yersinia ruckeri in the 2% FLP group was higher than the other treatments. Furthermore, the serum bactericidal activity against P. aeruginosa (ATCC 27853) only observed in fish fed on the 1 and 2% FLP-supplemented diets. The skin mucosal bactericidal activity and inhibitory effects increased with enhancing the FLP level in diet against E. coli, K. pneumonia, Y. ruckeri and A. hydrophila in a dose response manner. Moreover, the skin mucosal bactericidal activity against S. aureus only observed in fish fed on 1 and 2% FLP-supplemented diets. The weight gain values in the 1 and 2% FLP groups were higher than the other treatments (P < 0.05). Feed conversion ratio (FCR) improved with increasing FLP level in diet in a dose-response manner (P < 0.05). By considering serum and mucosal bactericidal activities against different pathogenic bacteria, the supplementation of 2% FLP in diet is recommended for C. carpio during grow-out phase.

进行了一项为期56天的研究，以检验饮食中蕨类（铁线蕨）叶粉（FLP）的分级水平（0（对照），0.5、1和2％）对鲤鱼免疫能力和生长的影响（鲤鱼，初始重量＝ 20g）。1％和2％FLP组的血清总免疫球蛋白含量和溶菌酶活性较其他组显着增加（P  <0.05）。皮肤粘膜溶菌酶的活性随着饮食中FLP水平的增加而呈剂量反应。以FLP补充饮食喂养的鱼比对照组的皮肤黏膜超氧化物歧化酶活性更高（P <0.05）。但是，血清抗氧化酶不受日粮蕨类食物的影响（P> 0.05）。饮食中针对金黄色葡萄球菌，大肠杆菌（EHEC ATCC 43895），大肠杆菌（CI），铜绿假单胞菌，肺炎克雷伯菌和亲水性嗜水气单胞菌的FLP水平提高，血清对人和鱼类病原体的杀菌活性增加。2％FLP组的血清抗鼠李斯特氏菌的活性高于其他治疗方法。此外，血清对铜绿假单胞菌的杀菌活性（ATCC 27853）仅在以1％和2％FLP补充饮食喂养的鱼中观察到。皮肤粘膜杀菌活性和抑制作用与增强对饮食的FLP水平提高的大肠杆菌，肺炎克雷伯菌，Y.耶尔森和嗜水以剂量响应的方式。而且，仅在饲喂添加了1％和2％FLP的饲料的鱼中观察到了针对金黄色葡萄球菌的皮肤粘膜杀菌活性。1％和2％FLP组的体重增加值高于其他治疗组（P  <0.05）。饲料中的转化率（FCR）随着日粮中FLP含量的增加而呈剂量反应方式（P <0.05）。考虑到血清和粘膜对不同病原菌的杀菌活性，建议在鲤鱼成长期期间在日粮中添加2％FLP 。