当前位置: X-MOL 学术Exp. Cell Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
TIGIT presents earlier expression dynamic than PD-1 in activated CD8+ T cells and is upregulated in non-small cell lung cancer patients.
Experimental Cell Research ( IF 3.3 ) Pub Date : 2020-09-02 , DOI: 10.1016/j.yexcr.2020.112260
Feng Hu 1 , Weiqin Wang 2 , Chuanhua Fang 2 , Chong Bai 3
Affiliation  

CD8+ T cells are considered a critical component of antitumor immunity. However, tumor-infiltrating CD8+ T cells may express more than one checkpoint molecules that have the potential to inhibit effector responses alone or cooperatively. Here, we focused on the expression dynamic of TIGIT and PD-1 in CD8+ T cells. TIGIT+ subset presented significantly higher PD-1 expression than TIGIT subset in circulating CD8+ T cells. The expression dynamic of TIGIT and PD-1 was then tracked. In total CD8+ T cells, TIGIT mRNA increased more rapidly than PD-1 mRNA, and TIGIT+ CD8+ T cells upregulated PD-1 more rapidly than TIGIT CD8+ T cells. Next, 24-h-stimulated CD8+ T cells were re-sorted into TIGIT+ and TIGIT subsets, and the TIGIT+ cells that came from TIGIT cells also presented significantly more rapid PD-1 induction than persistent TIGIT CD8+ T cells. In non-small cell lung cancer (NSCLC) patients, the expression of PD-1 was more enriched in TIGIT+ cells than in TIGIT cells in both circulating CD8+ T cells and tumor-infiltrating CD8+ T cells. Function analysis revealed that TIGIT+ CD8 T cells presented lower interferon-gamma, perforin 1, and granzyme B upregulation than TIGIT CD8 T cells, especially in NSCLC patients. Overall, these data indicated that TIGIT presented earlier expression dynamic than PD-1 in activated CD8+ T cells and was upregulated in NSCLC patients.



中文翻译:

TIGIT在活化的CD8 + T细胞中比PD-1表现出更早的动态表达,并且在非小细胞肺癌患者中被上调。

CD8 + T细胞被认为是抗肿瘤免疫力的重要组成部分。但是,浸润肿瘤的CD8 + T细胞可能表达一种以上的检查点分子,这些分子有可能单独或协同抑制效应子反应。在这里,我们专注于TIGIT和PD-1在CD8 + T细胞中的表达动态。TIGIT +子集呈现显著更高PD-1的表达比TIGIT -在循环CD8子+ T细胞。然后跟踪TIGIT和PD-1的表达动态。在CD8 + T细胞总数中,TIGIT mRNA的增长速度比PD-1 mRNA和TIGIT + CD8 +T细胞比TIGIT - CD8 + T细胞更快地上调PD-1 。接下来,24小时的刺激的CD8 + T细胞重新分为TIGIT +和TIGIT -子集,和TIGIT +从TIGIT传来细胞-细胞也呈现PD-1诱导比持续TIGIT显著更快速- CD8 + ŧ细胞。在非小细胞肺癌(NSCLC)患者,表达PD-1更富含TIGIT +细胞中比在TIGIT -细胞在这两个循环CD8 + T细胞和肿瘤浸润性CD8 +T细胞。功能分析表明,TIGIT + CD8 T细胞的干扰素-γ,穿孔素1和颗粒酶B的上调低于TIGIT - CD8 T细胞,尤其是在NSCLC患者中。总体而言,这些数据表明,在活化的CD8 + T细胞中,TIGIT的表达动力学比PD-1早,并且在NSCLC患者中表达上调。

更新日期:2020-09-11
down
wechat
bug