The use of natural biocompatible drugs is highly desirable in wound dressing compared to synthetic chemicals. Oregano essential oil (OEO) is a promising natural compound with marked antibacterial, antioxidant and anti-inflammatory activities. The topical delivery of OEO may result in lower therapeutic efficacy and irritation to the skin. Moreover, OEO is a volatile compound results in instability as well. To overcome these drawbacks, we successfully encapsulated OEO in Poly (L-lactide-co-caprolactone) (PLCL)/ Silk fibroin (SF) nanofibers membrane (NF) and achieved the encapsulation efficiency (%) up to 59.14 ± 0.58. The fabricated membranes were undergone through physicochemical as well as biological evaluation. SEM characterization revealed that OEO could be successfully encapsulated maintaining a smooth profile of nanofibers. The biocompatibility of the NF membrane was confirmed by cytotoxicity assay. Antibacterial results indicated that OEO containing nanofibrous membranes are highly active against both gram-positive and gram-negative bacteria. The result revealed that 5% is the optimized concentration of OEO capable to completely inhibit bacterial growth. Moreover, the NF membranes were evaluated for their in vivo wound healing potential. The results confirmed that OEO containing NF membrane is not only capable to accelerate the wound contraction but also enhances the quality of wound healing confirmed through histology analysis. H&E and Masson’s trichrome staining indicated the neo-epithelialization, granulation tissue formation, angiogenesis, and collagen deposition in a group treated with PLCL/SF/5% OEO. Based on the physicochemical and biological evaluation, PLCL/SF/5% OEO NF membrane can be considered as a potential wound dressing candidate.

与合成化学品相比，在伤口敷料中非常需要使用天然生物相容性药物。牛至精油（OEO）是一种很有前途的天然化合物，具有显着的抗菌，抗氧化和消炎活性。OEO的局部递送可能导致较低的治疗功效和对皮肤的刺激。此外，OEO是导致不稳定的挥发性化合物。为了克服这些缺点，我们成功地将OEO封装在聚（L-丙交酯-己内酯）（PLCL）/丝素蛋白（SF）纳米纤维膜（NF）中，并实现了高达59.14±0.58的封装效率（％）。所制作的膜经过了物理化学和生物学评估。SEM表征表明，OEO可以成功封装，保持纳米纤维的光滑轮廓。NF膜的生物相容性通过细胞毒性试验得以证实。抗菌结果表明，含有OEO的纳米纤维膜对革兰氏阳性和革兰氏阴性细菌均具有很高的活性。结果表明，5％的OEO浓度能够完全抑制细菌生长。此外，评估了NF膜的体内伤口愈合潜力。结果证实，包含OEO的NF膜不仅能够加速伤口收缩，而且通过组织学分析证实可以提高伤口愈合的质量。H＆E和Masson的三色染色表明，在用PLCL / SF / 5％OEO处理的组中，新上皮形成，肉芽组织形成，血管生成和胶原蛋白沉积。根据理化和生物学评估，