Theranostics including imaging and therapy has become an advanced trend for cancer treatment. It is of great significance to develop new theranostics agent for precision medicine research. In this regard, structurally precise metal nanoclusters (NCs) with ultra-small diameter and potential theranostic functions are no doubt to be a priority. In this study, we firstly investigated the photothermal and photodynamic properties of structurally precise oil-soluble bimetallic NCs (Pt₁Ag₂₈) and tactfully solubilize them into the hydrophobic inner cavity of biodegradable amphiphilic chitosan derivative (ACD) micelles via hydrophobic interaction. Positively charged Pt₁Ag₂₈@ACD with suitable particle size (∼60 nm) could not only realize effectively passive targeted delivery, but also be easily uptaken by negatively charged cancer cells for increasing targeting effect. Further, the aggregation induced emission fluorescence imaging (AIE FI)-guided enhanced photothermal/photodynamic therapy (PTT/PDT) on tumor is finally nicely achieved by the micelle solubilization of ACD micelles to Pt₁Ag₂₈ NCs, which were demonstrated by in vitro and in vivo experimental results. This theranostic platform overcomes the difficulty of lipophilic NCs entering water-phase organisms, provides enlightenment for the universal study of the interaction mechanism between organisms and oil-soluble NCs at the atomic level in future precision medicine research.

包括成像和治疗在内的治疗诊断学已经成为癌症治疗的先进趋势。开发新型的治疗药物对精密医学研究具有重要意义。在这一点上，具有超小直径和潜在治疗功能的结构精确的金属纳米簇（NC）无疑是当务之急。在这项研究中，我们首先研究了结构精确的油溶性双金属NCs（Pt ₁ Ag ₂₈）的光热和光动力学性质，并通过疏水作用将它们巧妙地溶解在可生物降解的两亲性壳聚糖衍生物（ACD）胶束的疏水性内腔中。带正电的Pt ₁ Ag ₂₈具有合适粒径（约60 nm）的@ACD不仅可以有效地实现被动靶向递送，而且很容易被带负电荷的癌细胞摄取以增加靶向作用。此外，通过将ACD胶束增溶到Pt ₁ Ag ₂₈ NCs上，胶束最终被很好地实现了聚集诱导发射荧光成像（AIE FI）指导的肿瘤增强光热/光动力疗法（PTT / PDT）。和体内实验结果。该治疗学平台克服了亲脂性NCs进入水相生物体的困难，为今后在精密医学研究中从原子水平对生物体与油溶性NCs相互作用机理的普遍研究提供了启示。