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Optical Properties of Hyperosmotic Agents for Immersion Clearing of Tissues in Terahertz Spectroscopy
Optics and Spectroscopy ( IF 0.8 ) Pub Date : 2020-09-02 , DOI: 10.1134/s0030400x20070279
G. R. Musina , A. A. Gavdush , N. V. Chernomyrdin , I. N. Dolganova , V. E. Ulitko , O. P. Cherkasova , V. N. Kurlov , G. A. Komandin , I. V. Zhivotovskii , V. V. Tuchin , K. I. Zaytsev

Abstract

We measured the transmission spectra of the most common hyperosmotic agents, such as pure glycerol, propylene glycol (PG), dimethyl sulfoxide (DMSO), polyethylene glycol (PEG) with molecular weights of 200, 300, 400, and 600 Da, their aqueous solutions, and aqueous solutions of sucrose, glucose, fructose, and dextran 40 and 70. The experiments were carried out using a THz pulsed spectrometer with an evacuated measuring compartment to eliminate the effect of water vapor on spectral measurements. We reconstructed the dielectric characteristics of hyperosmotic agents in the spectral range from 0.1 to 2.5 THz and plotted a dependence of the amplitude absorption coefficient on the concentration of the considered agents at a frequency of 0.5 THz. The results are useful for selecting optimal agents for immersion optical clearing in the THz range.


中文翻译:

太赫兹光谱中浸没清除组织的高渗透剂的光学性质

摘要

我们测量了分子量为200、300、400和600 Da的纯甘油,丙二醇(PG),二甲基亚砜(DMSO),聚乙二醇(PEG)等最常见的高渗剂的透射光谱溶液,蔗糖,葡萄糖,果糖和右旋糖酐40和70的水溶液。使用THz脉冲光谱仪和抽空的测量室进行实验,以消除水蒸气对光谱测量的影响。我们在0.1到2.5 THz的光谱范围内重构了高渗透剂的介电特性,并绘制了在0.5 THz频率下振幅吸收系数对所考虑的试剂浓度的依赖性。该结果对于选择在THz范围内进行浸没式光学清除的最佳试剂很有用。
更新日期:2020-09-02
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