Thioredoxin (Trx) proteins are essential for the maintenance of cellular redox balance through thiol/disulfide exchange modification. In Arabidopsis, the Trx superfamily consists of multiple protein isotypes distributed in most cellular compartments. Although the functions of chloroplastic and cytosolic Trxs have been investigated in plants, the physiological role of nuclear Trx proteins remains elusive. Nucleoredoxin (NRX) is a nuclear Trx first identified in eukaryotic organisms. Arabidopsis possesses two NRX genes (AtNRX1 and AtNRX2), and the function of AtNRX2 has not been elucidated to date. In this study, we characterized the function of AtNRX2 using the atnrx2 knockout mutant, based on its comparison with the atnrx1 mutant. In atnrx2 knockout mutant plants, trichome number was significantly reduced compared with the wild type (WT; Col-0) and the atnrx1 mutant. In response to JA induction of trichome, trichome formation was markedly diminished in the atnrx2 mutant. In addition, expression levels of genes involved in trichome formation were reduced in the atnrx2 mutant compared with the WT and atnrx1 mutant. Overall, our results suggest that AtNRX2 plays a physiological role in JA-mediated trichome formation in Arabidopsis.

硫氧还蛋白（Trx）蛋白对于通过硫醇/二硫键交换修饰来维持细胞氧化还原平衡至关重要。在拟南芥中，Trx超家族由分布在大多数细胞区室中的多种蛋白质同种型组成。尽管已经在植物中研究了叶绿体和胞质Trx的功能，但核Trx蛋白的生理作用仍然难以捉摸。Nucleoredoxin（NRX）是最早在真核生物中鉴定的核Trx。拟南芥具有两个NRX基因（AtNRX1和AtNRX2），并且迄今为止尚未阐明AtNRX2的功能。在这项研究中，我们使用atnrx2表征了AtNRX2的功能基因敲除突变体，基于与atnrx1突变体的比较。与野生型（WT; Col-0）和atnrx1突变体相比，在atnrx2敲除突变体植物中，毛状体数显着减少。为了响应JA对毛状体的诱导，在atnrx2突变体中毛状体的形成显着减少。另外，与WT和atnrx1突变体相比，atnrx2突变体中与毛状体形成有关的基因的表达水平降低。总体而言，我们的结果表明，AtNRX2在拟南芥中JA介导的毛状体形成中起着生理作用。