Helicobacter pylori, present in the stomach lining, is a Gramnegative bacterium that causes various gastrointestinal diseases, including gastritis and peptic ulcers. Propolis is a natural resinous substance collected from a variety of plants, and contains several natural bioactive substances. The aim of this study was to investigate the anti-inflammatory and antioxidative effects of Korean propolis on H. pylori-induced damage in the human adenocarcinoma gastric cell line. The propolis used in this study was obtained from the Korea Beekeeping Association in South Korea. The expression of pro-inflammatory interleukins (ILs), such as IL-8, IL-12, IL-1β, tumor necrosis factor alpha, cyclooxygenase-2, and inducible nitric oxide synthase, which was increased after H. pylori infection, significantly decreased in a dose-dependent manner upon pretreatment with Korean propolis, because of the suppression of mitogen-activated protein kinases and nuclear factor kB pathway. The anti-oxidative activity of propolis was assessed using the 2,2-diphenyl-1-picrylhydrazyl hydrate free radical assay. Korean propolis showed significant anti-oxidative effects via reactive oxygen species scavenging. In addition, pretreatment with Korean propolis upregulated the expression of anti-oxidant enzymes through Nrf2 signaling activation. These findings indicate that the use of Korean propolis, which has anti-inflammatory and anti-oxidative effects, can be promising for the prevention of H. pylori-induced gastric damage.

胃壁中存在的幽门螺杆菌是一种革兰氏阴性菌，会引起各种胃肠道疾病，包括胃炎和消化性溃疡。蜂胶是从多种植物中收集的天然树脂物质，并且含有几种天然生物活性物质。这项研究的目的是研究韩国蜂胶对幽门螺杆菌诱导的人腺癌胃细胞系损伤的抗炎和抗氧化作用。这项研究中使用的蜂胶是从韩国韩国养蜂协会获得的。幽门螺杆菌感染后促炎性白介素（ILs）的表达增加，例如IL-8，IL-12，IL-1β，肿瘤坏死因子α，环氧合酶-2和诱导型一氧化氮合酶。由于抑制了促分裂原活化的蛋白激酶和核因子kB通路，在用韩国蜂胶进行预处理后，感染的感染以剂量依赖的方式显着降低。蜂胶的抗氧化活性使用2，2-二苯基-1-吡咯肼基水合物自由基测定法进行评估。韩国蜂胶通过清除活性氧来显示出显着的抗氧化作用。此外，用韩国蜂胶进行的预处理通过Nrf2信号激活上调了抗氧化酶的表达。这些发现表明，使用具有抗炎和抗氧化作用的韩国蜂胶可以预防幽门螺杆菌引起的胃部损伤。