Studies have shown that lobetyolin (LBT), a component of traditional Chinese herbal medicine, has many very good biological activities and functions. However, its side effects and toxicities have not been evaluated adequately. In this work, we investigated the effects of LBT in transgenic zebrafish. LBT treatments promoted angiogenesis and led to vascular morphological malformation in zebrafish embryos, although they were normal in appearance. Interestingly, our results indicated that LBT has a function of promoting nerve growth in the embryonic stage of zebrafish. We also treated the zebrafish with combretastatin A-4 (which resulted in neuronal apoptosis) and LBT simultaneously and found that LBT promoted nerve growth at higher concentrations. Taken together, our findings clearly display that LBT efficiently promotes angiogenesis, leading to vascular morphological malformation, has low toxicity, and notably promotes neuronal development in zebrafish.

研究表明，传统的中草药成分洛贝索林（LBT）具有许多非常好的生物学活性和功能。但是，其副作用和毒性尚未得到充分评估。在这项工作中，我们调查了LBT在转基因斑马鱼中的作用。LBT治疗促进斑马鱼胚胎中的血管生成并导致血管形态畸形，尽管它们的外观正常。有趣的是，我们的结果表明LBT具有促进斑马鱼胚胎期神经生长的功能。我们还用康普他汀A-4（导致神经元凋亡）和LBT同时处理了斑马鱼，发现LBT在较高浓度下促进神经生长。综上所述，我们的发现清楚地表明，LBT有效促进血管生成，