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Identification of Monohexosylceramides From Euglena gracilis by Electrospray Ionization Mass Spectrometry
Natural Product Communications ( IF 1.5 ) Pub Date : 2020-08-31 , DOI: 10.1177/1934578x20942351
Nairui Zhang 1 , Kanae Kohama 1 , Miyuki Miyagawa 1 , Moe Mansho 1 , Ryota Sugimoto 2, 3 , Ayaka Nakashima 2, 3 , Kengo Suzuki 2, 3 , Hiroshi Kitagaki 1
Affiliation  

To date, the monohexosylceramides present in the eukaryotic alga Euglena gracilis has not been reported. In this study, we extracted and purified a lipid fraction that eluted similarly to other reported monohexosylceramides. The structural determination of the lipid fraction revealed a monohexosylceramide (m/z = 889.5 and a loss of m/z = 162), corresponding to the formula C54H99O8N having moieties corresponding to a monohexose (C6H12O6), a 9-methyl-4,8-sphingadienine (C19H37O2N), and a nonacosanoic acid with 2 double bonds (C29H54O2). This is the first report of the isolation of monohexosylceramides from E. gracilis and will promote its utilization in functional foods and cosmetics.



中文翻译:

电喷雾电离质谱法鉴定细叶藻中的单己基神经酰胺

迄今为止,尚未报道存在于真核藻Euglena gracilis中的单己糖基神经酰胺。在这项研究中,我们提取并纯化了与其他报道的单己糖基神经酰胺相似洗脱的脂质级分。脂质部分的结构测定显示一己糖基神经酰胺(m / z = 889.5,损失m / z = 162),对应于具有对应于一己糖(C 6 H 12 O)的部分的式C 54 H 99 O 8 N 6),9-甲基-4,8-​​鞘二烯胺(C 19 H 37 O 2N)和具有2个双键的非二十烷酸(C 29 H 54 O 2)。这是monohexosylceramides的隔离的第一份报告眼虫,将宣传使用的功能性食品和化妆品。

更新日期:2020-09-01
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