Tag-Free, Temperature Dependent Infrared Spectra of the GFP Chromophore: Revisiting the Question of Isomerism.,The Journal of Physical Chemistry A

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Tag-Free, Temperature Dependent Infrared Spectra of the GFP Chromophore: Revisiting the Question of Isomerism.
The Journal of Physical Chemistry A ( IF 2.7 ) Pub Date : 2020-08-31 , DOI: 10.1021/acs.jpca.0c07172
Wyatt Zagorec-Marks ₁ , Madison M Foreman ₁ , J Mathias Weber ₁

Affiliation

We report infrared spectra of a model chromophore of green fluorescent protein, prepared in an ion trap at temperatures ranging from 30 K to room temperature. We compare the changes in the infrared spectrum with predicted infrared spectra for the Z and E isomers of this molecule, and we confirm that the molecule exists as the Z isomer at low temperatures. We revisit the question whether or not it can thermally isomerize in the temperature range of this experiment, and we find no evidence for isomerization.

中文翻译：

GFP发色团的无标签，依赖温度的红外光谱：重新审视异构问题。

我们报告了绿色荧光蛋白模型发色团的红外光谱，该模型在离子阱中在30 K至室温的温度范围内制备。我们比较了该分子的Z和E异构体的红外光谱变化与预测的红外光谱，并确认该分子在低温下以Z异构体形式存在。我们重新讨论了它是否可以在该实验的温度范围内进行热异构化的问题，并且没有发现异构化的证据。

更新日期：2020-09-24

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