Efficient TEM characterization of cell–nanostructure interfacial interactions,Journal of the American Chemical Society

当前位置： X-MOL 学术 › J. Am. Chem. Soc. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Efficient TEM characterization of cell–nanostructure interfacial interactions
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2020-09-01 , DOI: 10.1021/jacs.0c05919
Stella Aslanoglou _{1,

2,

3} , Yaping Chen _{1,

2,

3} , Viola Oorschot _{4,

5} , Zlatan Trifunovic ₆ , Eric Hanssen ₆ , Koukou Suu ₇ , Nicolas H Voelcker _{1,

2,

3,

8,

9} , Roey Elnathan _{1,

2,

9}

Affiliation

Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC 3052, Australia.
Melbourne Centre for Nanofabrication, Victorian Node of the Australian National Fabrication Facility, 151 Wellington Road, Clayton, VIC 3168, Australia.
Commonwealth Scientific and Industrial Research Organisation (CSIRO), Clayton, VIC 3168, Australia.
Ramaciotti Centre for Cryo-Electron Microscopy, Monash University, 15 Innovation Walk, Clayton, VIC 3168, Australia.
Electron Microscopy Core Facility, European Molecular Biology Laboratory, Meyerhofstraße 1, Heidelberg 69117, Germany.
Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemmington Road, Parkville, VIC 3052, Australia.
Institute of Semiconductor and Electronics Technologies, ULVAC Inc., 1220-1 Suyama, Susono, Shizuoka 410-1231, Japan.
INM-Leibnitz Institute for New Materials, Campus D2 2, Saarbrücken 66123, Germany.
Department of Materials Science and Engineering, Monash University, 22 Alliance Lane, Clayton, VIC 3168, Australia.

Engineered nano-bio interfaces driven by tunable vertically configured nanostructures have recently emerged as a powerful tool for cellular manipulations and interrogations. Yet the interplay between substrate topography and cellular behavior is highly complex and not fully understood. A new experimental design is proposed that enables generation of ultrathin sections (lamellae) of cell-nanostructure imprints with minimal artefacts. We demonstrate the potential of such lamellae for efficient TEM characterization of interfacial interactions between adherent cells and vertically aligned Si nanostructures. This approach will advance understanding of cellular responses to extracellular biophysical and biochemical cues - which is likely to facilitate the design of improved cellular manipulation technologies.

中文翻译：

细胞-纳米结构界面相互作用的有效 TEM 表征

由可调垂直配置的纳米结构驱动的工程纳米生物界面最近已成为细胞操作和询问的强大工具。然而，基底地形和细胞行为之间的相互作用非常复杂，尚未完全了解。提出了一种新的实验设计，可以生成具有最少伪影的细胞纳米结构印记的超薄切片（薄片）。我们证明了这种薄片在粘附细胞和垂直排列的硅纳米结构之间的界面相互作用的有效 TEM 表征方面的潜力。这种方法将促进对细胞外生物物理和生化线索的细胞反应的理解——这可能有助于改进细胞操作技术的设计。

更新日期：2020-09-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11