Glomalin is a soil protein resembling heat shock protein (HSP) 60 and exerting high affinity to metals, causing retention of water in the environment and improving mechanical stability of soil. Currently, glomalin is determined in the soil or other samples by combination of autoclaving extraction and total protein determination typically by the Bradford method. In this paper, a piezoelectric biosensor was prepared to determine glomalin in a label-free measurement. The biosensor contained antibodies immobilized on quartz crystal microbalance (QCM), and the recognition layer was stabilized by iron oxide nanoparticles. The assay was tested on real soil samples and compared with the standard Bradford assay. Limit of detection of the assay was equal to 2.4 µg/g for a soil extract with a volume of 50 µl. The assay takes approximately half of an hour and was fully correlated to the Bradford assay. The biosensor had significant advantages than the other methods: it worked in a label-free mode and was fully applicable for practical samples.

中文翻译：

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含氧化铁纳米粒子的石英晶体微天平生物传感器对glomalin的免疫分析。

Glomalin是一种类似于热休克蛋白（HSP）60的土壤蛋白，对金属具有很高的亲和力，可将水保留在环境中并改善土壤的机械稳定性。当前，通常通过Bradford方法通过高压灭菌提取和总蛋白测定的组合来测定土壤或其他样品中的gloomalin。在本文中，准备了一种压电生物传感器来测定无标记测量中的gloomalin。该生物传感器包含固定在石英晶体微天平（QCM）上的抗体，并且识别层通过氧化铁纳米颗粒稳定。该测定法在真实的土壤样品上进行了测试，并与标准的布拉德福德测定法进行了比较。对于体积为50 µl的土壤提取物，该方法的检出限等于2.4  µg / g l。该测定大约需要半小时，并且与Bradford测定完全相关。该生物传感器比其他方法具有显着优势：它以无标签模式工作，完全适用于实际样品。